MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments
Martins, Pedro M. (Universidade do Porto. Instituto de Ciências Biomédicas Abel Salazar)
Navarro, Susanna (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Silva, Alexandra (Universidade do Porto. Instituto de Biologia Molecular e Celular and Instituto de Investigação e Inovação em Saúde)
Pinto, Maria F. (Universidade do Porto. Instituto de Biologia Molecular e Celular and Instituto de Investigação e Inovação em Saúde)
Sárkány, Zsuzsa (Universidade do Porto. Instituto de Biologia Molecular e Celular and Instituto de Investigação e Inovação em Saúde)
Figueiredo, Francisco (Universidade do Porto. Instituto de Ciências Biomédicas Abel Salazar)
Barbosa Pereira, Pedro José (Universidade do Porto. Instituto de Biologia Molecular e Celular and Instituto de Investigação e Inovação em Saúde)
Pinheiro, Francisca (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Bednarikova, Zuzana (Slovak Academy of Sciences. Department of Biophysics)
Burdukiewicz, Michal (Warsaw University of Technology. Faculty of Mathematics and Information Science)
Galzitskaya, Oxxana V. (Russian Academy of Sciences. Institute of Theoretical and Experimental Biophysics)
Gazova, Zuzana (Slovak Academy of Sciences. Department of Biophysics)
Gomes, Cláudio M. (Universidade de Lisboa. Departamento de Química e Bioquímica)
Pastore, Annalisa (King's College London. Maurice Wohl Clinical Neuroscience Institute)
Serpell, Louise C. (University of Sussex. School of Life Sciences)
Skrabana, Rostislav (Slovak Academy of Sciences. Institute of Neuroimmunology)
Smirnovas, Vytautas (Vilnius University. Institute of Biotechnology)
Ziaunys, Mantas (Vilnius University. Institute of Biotechnology)
Otzen, Daniel E. (Aarhus University. Department of Molecular Biology and Genetics)
Ventura, Salvador (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Macedo-Ribeiro, Sandra (Universidade do Porto. Instituto de Biologia Molecular e Celular and Instituto de Investigação e Inovação em Saúde)
Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular

Fecha:	2020
Resumen:	Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e. g. , for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.
Ayudas:	Ministerio de Economía y Competitividad BIO2016-78310-R
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Amyloid ; Reproducible data ; Protein ; Peptide ; Phase separation
Publicado en:	Frontiers in molecular neuroscience, Vol. 13 (November 2020) , art. 582488, ISSN 1662-5099

DOI: 10.3389/fnmol.2020.582488
PMID: 33328883

18 p, 1.7 MB

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