Immune cell subtypes and cytokines in lung tumor microenvironment : Influence of COPD
Tang, Jun (Institut Hospital del Mar d'Investigacions Mèdiques)
Ramis-Cabrer, Daniel (Institut Hospital del Mar d'Investigacions Mèdiques)
Curull Serrano, Víctor (Institut Hospital del Mar d'Investigacions Mèdiques)
Wang, Xuejie (Institut Hospital del Mar d'Investigacions Mèdiques)
Qin, Liyun (Institut Hospital del Mar d'Investigacions Mèdiques)
Mateu-Jiménez, Mercè (Institut Hospital del Mar d'Investigacions Mèdiques)
Duran-Jordà, Xavier (Institut Hospital del Mar d'Investigacions Mèdiques)
Pijuan, Lara (Institut Hospital del Mar d'Investigacions Mèdiques)
Rodríguez-Fuster, Alberto (Institut Hospital del Mar d'Investigacions Mèdiques)
Aguiló Espases, Rafael (Institut Hospital del Mar d'Investigacions Mèdiques)
Barreiro, Esther (Institut Hospital del Mar d'Investigacions Mèdiques)
Universitat Autònoma de Barcelona

Data:	2020
Resum:	Background: The immune microenvironment plays a role in tumorigenesis. Chronic Obstructive Pulmonary Disease (COPD) is an independent risk factor for lung cancer (LC). We hypothesized that immune profile characterized by T regulatory (Treg), natural killer (NK), and plasma cells, as well as interleukin (IL)-10 and interferon-gamma, may differ within tumors of LC patients with/without COPD. Methods: Treg (anti-CD3 and anti-forkhead boxP3 antibodies), NK (anti-NCR1 antibody), IgG (anti-CD138-IgG antibody), IgA (anti-CD138-IgA antibody) using immunohistochemistry, and both IL-10 and interferon-gamma (ELISA) were quantified in tumor and non-tumor specimens (thoracotomy for lung tumor resection) from 33 LC-COPD patients and 20 LC-only patients. Results: Immune profile in tumor versus non-tumor specimens: Treg cell counts significantly increased in tumors of both LC and LC-COPD patients, while in tumors of the latter group, IgG-secreting plasma cells significantly decreased and IL-10 increased. No significant differences were seen in levels of NK cells, IgA-secreting cells, IgA/IgG, or interferon-gamma. Immune profile in tumors of LC-COPD versus LC: No significant differences were observed in tumors between LC-COPD and LC patients for any study marker. Conclusions: Immune cell subtypes and cytokines are differentially expressed in lung tumors, and the presence of COPD elicited a decline in IgG-secreting plasma cell levels but not in other cell types.
Ajuts:	Instituto de Salud Carlos III FIS18-00075
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Cancers, Vol. 12 Núm. 5 (may 2020) , p. 1217, ISSN 2072-6694

DOI: 10.3390/cancers12051217
PMID: 32414037

15 p, 5.6 MB

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