Home > Articles > Published articles > Insights on the emerging biotechnology of histidine-rich peptides |
Date: | 2022 |
Abstract: | In the late 70's, the discovery of the restriction enzymes made possible the biological production of functional proteins by recombinant DNA technologies, a fact that largely empowered both biotechnological and pharmaceutical industries. Short peptides or small protein domains, with specific molecular affinities, were developed as purification tags in downstream processes to separate the target protein from the culture media or cell debris, upon breaking the producing cells. Among these tags, and by exploiting the interactivity of the imidazole ring of histidine residues, the hexahistidine peptide (H6) became a gold standard. Although initially used almost exclusively in protein production, H6 and related His-rich peptides are progressively proving a broad applicability in novel utilities including enzymatic processes, advanced drug delivery systems and diagnosis, through a so far unsuspected adaptation of their binding capabilities. In this context, the coordination of histidine residues and metals confers intriguing functionalities to His-rich sequences useable in the forward-thinking design of protein-based nano- and micro-materials and devices, through strategies that are comprehensively presented here. |
Grants: | Ministerio de Ciencia e Innovación BIO2016-76063-R Ministerio de Ciencia e Innovación PID2019-105416RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 Instituto de Salud Carlos III PI20/0040 Agència de Gestió d'Ajuts Universitaris i de Recerca 2019/FI_B00352 Ministerio de Ciencia e Innovación FPU18/04615 Ministerio de Ciencia e Innovación CP19/00028 |
Note: | Altres ajuts: acords transformatius de la UAB |
Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Language: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Published in: | Biotechnology advances, Vol. 54 (January-February 2022) , art. 107817, ISSN 0734-9750 |
15 p, 5.7 MB |