Web of Science: 31 citations, Scopus: 34 citations, Google Scholar: citations,
Insights on the emerging biotechnology of histidine-rich peptides
López-Laguna, Hèctor (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Voltà-Durán, Eric (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Parladé Molist, Eloi (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Unzueta Elorza, Ugutz (Institut d'Investigació Biomèdica Sant Pau)

Date: 2022
Abstract: In the late 70's, the discovery of the restriction enzymes made possible the biological production of functional proteins by recombinant DNA technologies, a fact that largely empowered both biotechnological and pharmaceutical industries. Short peptides or small protein domains, with specific molecular affinities, were developed as purification tags in downstream processes to separate the target protein from the culture media or cell debris, upon breaking the producing cells. Among these tags, and by exploiting the interactivity of the imidazole ring of histidine residues, the hexahistidine peptide (H6) became a gold standard. Although initially used almost exclusively in protein production, H6 and related His-rich peptides are progressively proving a broad applicability in novel utilities including enzymatic processes, advanced drug delivery systems and diagnosis, through a so far unsuspected adaptation of their binding capabilities. In this context, the coordination of histidine residues and metals confers intriguing functionalities to His-rich sequences useable in the forward-thinking design of protein-based nano- and micro-materials and devices, through strategies that are comprehensively presented here.
Grants: Ministerio de Ciencia e Innovación BIO2016-76063-R
Ministerio de Ciencia e Innovación PID2019-105416RB-I00
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229
Instituto de Salud Carlos III PI20/0040
Agència de Gestió d'Ajuts Universitaris i de Recerca 2019/FI_B00352
Ministerio de Ciencia e Innovación FPU18/04615
Ministerio de Ciencia e Innovación CP19/00028
Note: Altres ajuts: acords transformatius de la UAB
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès
Document: Article ; recerca ; Versió publicada
Published in: Biotechnology advances, Vol. 54 (January-February 2022) , art. 107817, ISSN 0734-9750

DOI: 10.1016/j.biotechadv.2021.107817
PMID: 34418503


15 p, 5.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles

 Record created 2021-09-06, last modified 2023-11-29



   Favorit i Compartir