The activity of alkaline phosphatase in breast cancer exosomes simplifies the biosensing design
Lima Moura, Silio (Universitat Autònoma de Barcelona. Departament de Química)
Pallarès-Rusiñol, Arnau (Universitat Autònoma de Barcelona. Departament de Química)
Sappia, Luciano (Universitat Autònoma de Barcelona. Departament de Química)
Martí, Mercè (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química)

Date:	2022
Abstract:	This work addresses a biosensor combining the immunomagnetic separation and the electrochemical biosensing based on the intrinsic ALP activity of the exosomes. This approach explores for the first time two different types of biomarkers on exosomes, in a unique biosensing device combining two different biorecognition reaction: immunological and enzymatic. Besides, the intrinsic activity of alkaline phosphatase (ALP) in exosomes as a potential biomarker of carcinogenesis as well as osseous metastatic invasion is also explored. To achieve that, as an in vitro model, exosomes from human fetal osteoblasts are used. It is demonstrated that the electrochemical biosensor improves the analytical performance of the gold standard colorimetric assay for the detection of ALP activity in exosomes, providing a limit of detection of 4. 39 mU L-1, equivalent to 105 exosomes μL-1. Furthermore, this approach is used to detect and quantify exosomes derived from serum samples of breast cancer patients. The electrochemical biosensor shows reliable results for the differentiation of healthy donors and breast cancer in- dividuals based on the immunomagnetic separation using specific epithelial biomarkers CD326 (EpCAM) com- bined with the intrinsic ALP activity electrochemical readout.
Grants:	Ministerio de Economía y Competitividad BIO2016-75751-R Agencia Estatal de Investigación PID2019-106625RB-I00 Ministerio de Educación, Cultura y Deporte FPU16/01579
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Exosomes ; Alkaline phosphatase ; Breast cancer ; Liquid biopsy ; Immunomagnetic separation ; Electrochemical biosensor
Published in:	Biosensors & bioelectronics, Vol. 198 (February 2022) , art. 113826, ISSN 1873-4235

DOI: 10.1016/j.bios.2021.113826
PMID: 34891059

7 p, 4.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles