Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
Perea, Claudia (United States Department of Agriculture)
Ciaravino, Giovanna (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Stuber, Tod (United States Department of Agriculture)
Thacker, Tyler C. (United States Department of Agriculture)
Robbe-Austerman, Suelee (United States Department of Agriculture)
Allepuz Palau, Alberto (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Pérez de Val, Bernat (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Data:	2021
Resum:	The high-resolution WGS analyses of MTBC strains have provided useful insight for determining sources of infection for animal tuberculosis. In Spain, tuberculosis in livestock is caused by Mycobacterium bovis and Mycobacterium caprae, where wildlife reservoirs play an important role. We analyzed a set of 125 M. bovis isolates obtained from livestock and wildlife from Catalonia to investigate strain diversity and identify possible sources and/or causes of infection. Whole-genome SNP profiles were used for phylogenetic reconstruction and pairwise SNP distance analysis. Additionally, SNPs were investigated to identify virulence and antimicrobial resistance factors to investigate clade-specific associations. Putative transmission clusters (≤12 SNPs) were identified, and associated epidemiological metadata were used to determine possible explanatory factors for transmission. M. bovis distribution was heterogeneous, with 7 major clades and 21 putative transmission clusters. In order of importance, the explanatory factors associated were proximity and neighborhood, residual infection, livestock-wildlife interaction, shared pasture, and movement. Genes related to lipid transport and metabolism showed the highest number of SNPs. All isolates were pyrazinamide resistant, and five were additionally resistant to isoniazid, but no clade-specific associations could be determined. Our findings highlight the importance of high-resolution molecular surveillance to monitor bovine tuberculosis dynamics in a low-prevalence setting.
Ajuts:	Ministerio de Ciencia e Innovación AGL2013-49159-C2-1-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Whole-genome sequencing ; SNP ; Mycobacterium bovis ; Bovine tuberculosis ; Catalonia ; Northeastern Spain
Publicat a:	Microorganisms, Vol. 9 Núm. 8 (august 2021) , p. 1629, ISSN 2076-2607

DOI: 10.3390/microorganisms9081629
PMID: 34442709

24 p, 1.5 MB

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