CD32 expression is associated to T-cell activation and is not a marker of the HIV-1 reservoir
Badia, Roger (IrsiCaixa)
Ballana, Ester (IrsiCaixa)
Castellví, Marc (IrsiCaixa)
García Vidal, Edurne (IrsiCaixa)
Pujantell, Maria (IrsiCaixa)
Clotet Sala, Bonaventura (IrsiCaixa)
Prado, Julia G. (IrsiCaixa)
Puig, Jordi (IrsiCaixa)
Martinez, Miguel Angel (IrsiCaixa)
Riveira Muñoz, Eva (IrsiCaixa)
Este, José (IrsiCaixa)
Universitat Autònoma de Barcelona. Departament de Medicina

Date:	2018
Abstract:	CD32 has been shown to be preferentially expressed in latently HIV-1-infected cells in an in vitro model of quiescent CD4 T cells. Here we show that stimulation of CD4+ T cells with IL-2, IL-7, PHA, and anti-CD3/CD28 antibodies induces T-cell proliferation, co-expression of CD32 and the activation of the markers HLA-DR and CD69. HIV-1 infection increases CD32 expression. 79. 2% of the CD32+/CD4+ T cells from HIV+ individuals under antiretroviral treatment were HLA-DR+. Resting CD4+ T cells infected in vitro generally results in higher integration of provirus. We observe no difference in provirus integration or replication-competent inducible latent HIV-1 in CD32+ or CD32- CD4+ T cells from HIV+ individuals. Our results demonstrate that CD32 expression is a marker of CD4+ T cell activation in HIV+ individuals and raises questions regarding the immune resting status of CD32+ cells harboring HIV-1 proviruses. CD32 has been previously shown to be expressed preferentially by CD4 T cells latently harbouring HIV-1. Here the authors show that CD32 expression in CD4 T cells is associated with T cell activation, is up-regulated by HIV-1 infection and importantly does not appear to represent an enriched cellular niche for latent HIV-1.
Grants:	Ministerio de Economía y Competitividad BFU2015-63800-R Ministerio de Economía y Competitividad PI15/00492 Ministerio de Economía y Competitividad PI16/00103 Instituto de Salud Carlos III PI17/00624
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Nature communications, Vol. 9 (july 2018) , ISSN 2041-1723

DOI: 10.1038/s41467-018-05157-w
PMID: 30013105

10 p, 894.8 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles