Malignant infarction of the middle cerebral artery in a porcine model. A pilot study
Arikan Abelló, Fuat (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Martínez-Valverde, Tamara (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Sánchez-Guerrero, Ángela (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Campos Martorell, Mireia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Esteves, Marielle (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gándara, Dario F. (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Torné, Ramon (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Castro, Lidia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Dalmau Bueno, Antoni (Animal Breeding and Genetics Program (Girona))
Tibau, Joan (Animal Breeding and Genetics Program, Monells (Girona))
Sahuquillo Barris, Juan (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Data:	2017
Resum:	Interspecies variability and poor clinical translation from rodent studies indicate that large gyrencephalic animal stroke models are urgently needed. We present a proof-of-principle study describing an alternative animal model of malignant infarction of the middle cerebral artery (MCA) in the common pig and illustrate some of its potential applications. We report on metabolic patterns, ionic profile, brain partial pressure of oxygen (PtiO), expression of sulfonylurea receptor 1 (SUR1), and the transient receptor potential melastatin 4 (TRPM4). A 5-hour ischemic infarct of the MCA territory was performed in 5 2. 5-to-3-month-old female hybrid pigs (Large White x Landrace) using a frontotemporal approach. The core and penumbra areas were intraoperatively monitored to determine the metabolic and ionic profiles. To determine the infarct volume, 2,3,5-triphenyltetrazolium chloride staining and immunohistochemistry analysis was performed to determine SUR1 and TRPM4 expression. PtiO monitoring showed an abrupt reduction in values close to 0 mmHg after MCA occlusion in the core area. Hourly cerebral microdialysis showed that the infarcted tissue was characterized by reduced concentrations of glucose (0. 03 mM) and pyruvate (0. 003 mM) and increases in lactate levels (8. 87mM), lactate-pyruvate ratio (4202), glycerol levels (588 μM), and potassium concentration (27. 9 mmol/L). Immunohistochemical analysis showed increased expression of SUR1-TRPM4 channels. The aim of the present proof-of-principle study was to document the feasibility of a large animal model of malignant MCA infarction by performing transcranial occlusion of the MCA in the common pig, as an alternative to lisencephalic animals. This model may be useful for detailed studies of cerebral ischemia mechanisms and the development of neuroprotective strategies.
Ajuts:	Instituto de Salud Carlos III FIS PI11/00700
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan aquestes es distribueixin sota la mateixa llicència que regula l'obra original i es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 12 (february 2017) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0172637
PMID: 28235044

21 p, 3.3 MB

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