Differential N- and O-glycosylation signatures of HIV-1 Gag virus-like particles and coproduced extracellular vesicles
Lavado-García, Jesús (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Zhang, Tao (Leiden University Medical Center. Center for Proteomics and Metabolomics)
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Wuhrer, Manfred (Leiden University Medical Center. Center for Proteomics and Metabolomics)

Data:	2022
Resum:	Human immunodeficiency virus 1 (HIV-1) virus-like particles (VLPs) are nanostructures derived from the self-assembly and cell budding of Gag polyprotein. Mimicking the native structure of the virus and being noninfectious, they represent promising candidates for the development of new vaccines as they elicit a strong immune response. In addition to this, the bounding membrane can be functionalized with exogenous antigens to target different diseases. Protein glycosylation depends strictly on the production platform and expression system used and the displayed glycosylation patterns may influence downstream processing as well as the immune response. One of the main challenges for the development of Gag VLP production bioprocess is the separation of VLPs and coproduced extracellular vesicles (EVs). In this study, porous graphitized carbon separation method coupled with mass spectrometry was used to characterize the N- and O- glycosylation profiles of Gag VLPs produced in HEK293 cells. We identified differential glycan signatures between VLPs and EVs that could pave the way for further separation and purification strategies to optimize downstream processing and move forward in VLP-based vaccine production technology.
Ajuts:	"la Caixa" Foundation LCF/BQ/ES17/11600003 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-898
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	HIV-1 ; N-glycans ; O-glycans ; Porous graphitized carbon ; Vaccine ; Virus-like particles
Publicat a:	Biotechnology and bioengineering, Vol. 119, Issue 5 (May 2022) , p. 1207-1221, ISSN 1097-0290

DOI: 10.1002/bit.28051
PMID: 35112714

15 p, 2.8 MB

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