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Pàgina inicial > Articles > Articles publicats > Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq |
Data: | 2023 |
Resum: | Fatty acids (FAs) play an essential role as mediators of cell signaling and signal transduction, affecting metabolic homeostasis and determining meat quality in pigs. However, FAs are transformed by the action of several genes, such as those encoding desaturases and elongases of FAs in lipogenic tissues. The aim of the current work was to identify candidate genes, biological processes, and pathways involved in the modulation of intramuscular FA profile from longissimus dorsi muscle. FA profile by gas chromatography of methyl esters and gene expression by RNA-Seq were determined in 129 Iberian × Duroc backcrossed pigs. An association analysis between the muscle transcriptome and its FA profile was performed, followed by a concordance and functional analysis. Overall, a list of well-known (e. g. , PLIN1, LEP, ELOVL6, SC5D, NCOA2, ACSL1, MDH1, LPL, LGALS12, TFRC, GOT1, and FBP1) and novel (e. g. , TRARG1, TANK, ENSSSCG00000011196, and ENSSSCG00000038429) candidate genes was identified, either in association with specific or several FA traits. Likewise, several of these genes belong to biological processes and pathways linked to energy, lipid, and carbohydrate metabolism, which seem determinants in the modulation of FA compositions. This study can contribute to elucidate the complex relationship between gene expression and FA profile in pig muscle. |
Ajuts: | Agència de Gestió d'Ajuts Universitaris i de Recerca 2019FI_B_00787-credential Agència de Gestió d'Ajuts Universitaris i de Recerca 2020FI_B_00225-credential Ministerio de Ciencia e Innovación RYC2019-027244-I Ministerio de Ciencia e Innovación AGL2017-82641-R Ministerio de Ciencia e Innovación AGL2014- 56369-C2 Instituto de Salud Carlos III PT17/0009/0019 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Gene expression ; Transcriptomics ; Gene expression analysis ; Gene expression profiling ; Fatty acids |
Publicat a: | Scientific reports, Vol. 13 (january 2023) , ISSN 2045-2322 |
12 p, 3.2 MB |