Establishment of a reproducible and minimally invasive ischemic stroke model in swine
Castaño Duque, Carlos (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Melià Sorolla, Marc (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
García Serran, Alexia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
De Gregorio-Rocasolano, Nuria (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
García-Sort, Maria Rosa (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Hernández-Pérez, María (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Valls-Carbó, Adrián (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pino, Osvaldo (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Grífols, Jordi (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Iruela-Sánchez, Alba (Canon Medical Systems)
Palomar-García, Alicia (Canon Medical Systems)
Puig, Josep (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Martí-Sistac, Octavi (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Dávalos, Antoni (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Gasull Dalmau, Teresa (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	The need for advances in the management/treatment options for ischemic stroke patients requires that upcoming preclinical research uses animals with more human-like brain characteristics. The porcine brain is considered appropriate, although the presence of the rete mirabile (RM) prevents direct catheterization of the intracranial arteries to produce focal cerebral ischemia. To develop a reproducible minimally invasive porcine stroke model, a guide catheter and guide wire were introduced through the femoral artery until reaching the left RM. Using the pressure cooker technique, Squid-12 embolization material was deposited to fill, overflow, and occlude the left RM, the left internal carotid artery, and left circle of Willis wing up to the origins of the middle cerebral arteries (MCAs), mimicking the occlusion produced in the filament model in rodents. Longitudinal multimodal cerebral MRI was conducted to assess the brain damage and cerebral blood supply. The technique we describe here occluded up to the origins of the MCAs in 7 of 8 swine, inducing early damage 90 minutes after occlusion that later evolved to a large cerebral infarction and producing no mortality during the intervention. This minimally invasive ischemic stroke model in swine produced reproducible infarcts and shows translational features common to human stroke.
Ajuts:	Instituto de Salud Carlos III PI18/01813 Instituto de Salud Carlos III PI21/01925 Ministerio de Economía y Competitividad RD16/0019/0020
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Neuroscience ; Stroke
Publicat a:	JCI Insight, Vol. 8 (april 2023) , ISSN 2379-3708

DOI: 10.1172/jci.insight.163398
PMID: 36853828

16 p, 7.7 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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