A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds : Uncovering Its Dual Antifungal and Hypoglycemic Properties
Cotabarren, Juliana (Universidad Nacional de la Plata. Centro de Investigación de Proteínas Vegetales)
Ozón, Brenda (Universidad Nacional de la Plata. Centro de Investigación de Proteínas Vegetales)
Claver, Santiago (Universidad Nacional de la Plata. Centro de Investigación de Proteínas Vegetales)
Geier, Florencia (Universidad Nacional de la Plata. Centro de Investigación de Proteínas Vegetales)
Rossotti, Martina (Universidad Nacional de la Plata. Centro de Investigación de Proteínas Vegetales)
Garcia-Pardo, Javier (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Obregón, Walter David (Universidad Nacional de la Plata. Centro de Investigación de Proteínas Vegetales)
Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular

Date:	2023
Abstract:	Fungal infections are a growing public health concern worldwide and the emergence of antifungal resistance has limited the number of therapeutic options. Therefore, developing novel strategies for identifying and developing new antifungal compounds is an active area of research in the pharmaceutical industry. In this study, we purified and characterized a trypsin protease inhibitor obtained from Yellow Bell Pepper (Capsicum annuum L. ) seeds. The inhibitor not only showed potent and specific activity against the pathogenic fungus Candida albicans, but was also found to be non-toxic against human cells. Furthermore, this inhibitor is unique in that it also inhibits α-1,4-glucosidase, positioning it as one of the first plant-derived protease inhibitors with dual biological activity. This exciting discovery opens new avenues for the development of this inhibitor as a promising antifungal agent and highlights the potential of plant-derived protease inhibitors as a rich source for the discovery of novel multifunctional bioactive molecules.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Antifungal ; Bioactive compound ; Bioactive peptide ; Capsicum annuum L. ; Candida albicans ; Protease ; Protease inhibitor ; Phytocystatin ; Trypsin inhibitor ; Natural product
Published in:	Pharmaceutics, Vol. 15, Issue 3 (March 2023) , art. 781, ISSN 1999-4923

DOI: 10.3390/pharmaceutics15030781
PMID: 36986642

13 p, 2.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles