Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing : bridging innate and adaptive cellular immune responses in camelid reservoirs
Rodon, Jordi (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Te, Nigeer (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Vergara-Alert, Júlia (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Bensaid, Albert (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)

Date:	2023
Abstract:	Middle East respiratory syndrome coronavirus (MERS-CoV) infection can cause fatal pulmonary inflammatory disease in humans. Contrarily, camelids and bats are the main reservoir hosts, tolerant for MERS-CoV replication without suffering clinical disease. Here, we isolated cervical lymph node (LN) cells from MERS-CoV convalescent llamas and pulsed them with two different viral strains (clades B and C). Viral replication was not supported in LN, but a cellular immune response was mounted. Reminiscent Th1 responses (IFN-γ, IL-2, IL-12) were elicited upon MERS-CoV sensing, accompanied by a marked and transient peak of antiviral responses (type I IFNs, IFN-λ3, ISGs, PRRs and TFs). Importantly, expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) or inflammasome components (NLRP3, CASP1, PYCARD) was dampened. The role of IFN-λ3 to counterbalance inflammatory processes and bridge innate and adaptive immune responses in camelid species is discussed. Our findings shed light into key mechanisms on how reservoir species control MERS-CoV in the absence of clinical disease.
Grants:	European Commission 731014
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Camelids ; Cytokines ; Immune responses ; Llama ; Lymph node ; Lymphocyte ; Middle East respiratory syndrome coronavirus (MERS-CoV)
Published in:	Frontiers in immunology, Vol. 14 (june 2023) , ISSN 1664-3224

DOI: 10.3389/fimmu.2023.1205080
PMID: 37388723

8 p, 1.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles