Protein features instruct the secretion dynamics from metal-supported synthetic amyloids
Parladé Molist, Eloi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Sanchez, Julieta M (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
López-Laguna, Hèctor (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Unzueta Elorza, Ugutz (Institut d'Investigació Biomèdica Sant Pau)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2023
Abstract:	Hexahistidine-tagged proteins can be clustered by divalent cations into self-containing, dynamic protein depots at the microscale, which under physiological conditions leak functional protein. While such protein granules show promise in clinics as time-sustained drug delivery systems, little is known about how the nature of their components, that is, the protein and the particular cation used as cross-linker, impact on the disintegration of the material and on its secretory performance. By using four model proteins and four different cation formulations to control aggregation, we have here determined a moderate influence of the used cation and a potent impact of some protein properties on the release kinetics and on the final fraction of releasable protein. In particular, the electrostatic charge at the amino terminus and the instability and hydropathicity indexes determine the disintegration profile of the depot. These data offer clues for the fabrication of efficient and fully exploitable secretory granulesthat being biocompatible and chemically homogenous allow their tailored use as drug delivery platforms in biological systems.
Grants:	Agencia Estatal de Investigación PID2019-105416RB-I00 Agencia Estatal de Investigación PDC2022-133858-I00 Agencia Estatal de Investigación PID2020-116174RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2020/PANDE-00003 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00092 Instituto de Salud Carlos III PI20/00400 Instituto de Salud Carlos III CP19/00028 Ministerio de Sanidad y Consumo CB06/01/0014
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Recombinant proteins ; Microparticles ; Secretory amyloids ; Drug delivery system ; Time sustained drug release ; Building blocks
Published in:	International journal of biological macromolecules, Vol. 250 (October 2023) , art. 126164, ISSN 1879-0003
Related work:	Parladé Molist, Eloi; Sanchez, Julieta M.; Unzueta Elorza, Ugutz; Villaverde Corrales, Antonio; Vázquez Gómez, Esther, 2023, "Raw data to support the manuscript with the following scope: Protein releasability from synthetic secretory amyloids", CORA.Repositori de Dades de Recerca, V2 https://doi.org/10.34810/data613

DOI: 10.1016/j.ijbiomac.2023.126164
PMID: 37549767

8 p, 4.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles