Red Blood Cells and Endothelium Derived Circulating Extracellular Vesicles in Health and Chronic Heart Failure : A Focus on Phosphatidylserine Dynamics in Vesiculation
Suades, Rosa (Institut d'Investigació Biomèdica Sant Pau)
Vilella-Figuerola, Alba (Institut d'Investigació Biomèdica Sant Pau)
Padró, Teresa (Institut d'Investigació Biomèdica Sant Pau)
Mirabet Pérez, Sonia (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona. Departament de Medicina

Date:	2023
Abstract:	Circulating extracellular microvesicles (cEVs) are characterised by presenting surface antigens of parental cells. Since their biogenesis involves the translocation of phosphatidylserine (PS) from the inner to the outer leaflet of the plasma membrane, exposed PS has been considered as a recognition hallmark of cEVs. However, not all cEVs externalise PS. In this study, we have phenotypically and quantitatively characterised cEVs by flow cytometry, paying special attention to the proportions of PS in chronic heart failure patients (cHF; n = 119) and a reference non-HF group (n = 21). PS − -cEVs were predominantly found in both groups. Parental markers showed differential pattern depending on the PS exposure. Endothelium-derived and connexin 43-rich cEVs were mainly PS − -cEVs and significantly increased in cHF. On the contrary, platelet-derived cEVs were mostly PS + and were increased in the non-HF group. We observed similar levels of PS + - and PS − -cEVs in non-HF subjects when analysing immune cell-derived Evs, but there was a subset-specific difference in cHF patients. Indeed, those cEVs carrying CD45 +, CD29 +, CD11b +, and CD15 + were mainly PS + -cEVs, while those carrying CD14 +, CD3 +, and CD56 + were mainly PS − -cEVs. In conclusion, endothelial and red blood cells are stressed in cHF patients, as detected by a high shedding of cEVs. Despite PS + -cEVs and PS − -cEVs representing two distinct cEV populations, their release and potential function as both biomarkers and shuttles for cell communication seem unrelated to their PS content.
Grants:	Agencia Estatal de Investigación PID2019-107160RB-I00 Instituto de Salud Carlos III PI19/01687 Ministerio de Economía y Competitividad CB16/11/00411 European Commission. Horizon 2020 801370
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Annexin V ; Connexin 43 ; Endothelial cells ; Extracellular vesicles ; Heart failure ; Leukocytes ; Microvesicles ; Phosphatidylserine ; Platelets ; Red blood cells
Published in:	International journal of molecular sciences, Vol. 24, Num. 14 (july 2023) , ISSN 1422-0067

DOI: 10.3390/ijms241411824
PMID: 37511585

15 p, 3.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
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