An α-helical peptide-based plasmonic biosensor for highly specific detection of α-synuclein toxic oligomers
Giarola, Juliana Fátima (Institut Català de Nanociència i Nanotecnologia)
Santos, Jaime (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Ventura, Salvador (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Pallarès i Goitiz, Irantzu (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Lechuga, Laura M. (Institut Català de Nanociència i Nanotecnologia)

Date:	2024
Abstract:	Background: α-Synuclein (αS) aggregation is the main neurological hallmark of a group of neurodegenerative disorders, collectively referred to as synucleinopathies, of which Parkinson's disease (PD) is the most prevalent. αS oligomers are elevated in the cerebrospinal fluid (CSF) of PD patients, standing as a biomarker for disease diagnosis. However, methods for early PD detection are still lacking. We have recently identified the amphipathic 22-residue peptide PSMα3 as a high-affinity binder of αS toxic oligomers. PSMα3 displayed excellent selectivity and reproducibility, binding to αS toxic oligomers with affinities in the low nanomolar range and without detectable cross-reactivity with functional monomeric αS. Results: In this work, we leveraged these PSMα3 unique properties to design a plasmonic-based biosensor for the direct detection of toxic oligomers under label-free conditions. Significance and novelty: We describe the integration of the peptide in a lab-on-a-chip plasmonic platform suitable for point-of-care measurements of αS toxic oligomers in CSF samples in real-time and at an affordable cost, providing an innovative biosensor for PD early diagnosis in the clinic.
Grants:	Agencia Estatal de Investigación BIO2016-78310-R Agencia Estatal de Investigación BIO2017-91475-EXP Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00456 Agencia Estatal de Investigación PID2019-105017RB-I00 Agencia Estatal de Investigación CEX2021-001214-S Ministerio de Ciencia e Innovación FPU17/01157
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	α-synuclein ; Synucleinopathies diagnosis ; Parkinson's disease ; Oligomers ; Plasmonic biosensor
Published in:	Analytica chimica acta, Vol. 1304 (May 2024) , art. 342559, ISSN 1873-4324

DOI: 10.1016/j.aca.2024.342559

11 p, 4.8 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles