Vaccination with an HIV T-Cell Immunogen (HTI) Using DNA Primes Followed by a ChAdOx1-MVA Boost Is Immunogenic in Gut Microbiota-Depleted Mice despite Low IL-22 Serum Levels
Elizalde-Torrent, Aleix (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Borgognone, Alessandra (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Casadellà, Maria (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Romero-Martín, Luis (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Escribà, Tuixent (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Parera, Mariona (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Rosales-Salgado, Yaiza (Institut Germans Trias i Pujol)
Díaz-Pedroza, Jorge (Institut Germans Trias i Pujol)
Català-Moll, Francesc (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Noguera-Julian, Marc (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Brander, Christian (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Paredes, Roger (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Olvera, Alex (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Universitat Autònoma de Barcelona

Date:	2023
Abstract:	Despite the important role of gut microbiota in the maturation of the immune system, little is known about its impact on the development of T-cell responses to vaccination. Here, we immunized C57BL/6 mice with a prime-boost regimen using DNA plasmid, the Chimpanzee Adenovirus, and the modified Vaccinia Ankara virus expressing a candidate HIV T-cell immunogen and compared the T-cell responses between individuals with an intact or antibiotic-depleted microbiota. Overall, the depletion of the gut microbiota did not result in significant differences in the magnitude or breadth of the immunogen-specific IFNγ T-cell response after vaccination. However, we observed marked changes in the serum levels of four cytokines after vaccinating microbiota-depleted animals, particularly a significant reduction in IL-22 levels. Interestingly, the level of IL-22 in serum correlated with the abundance of Roseburia in the large intestine of mice in the mock and vaccinated groups with intact microbiota. This short-chain fatty acid (SCFA)-producing bacterium was significantly reduced in the vaccinated, microbiota-depleted group. Therefore, our results indicate that, although microbiota depletion reduces serum levels of IL-22, the powerful vaccine regime used could have overcome the impact of microbiota depletion on IFNγ-producing T-cell responses.
Grants:	European Commission. Horizon 2020 847943 European Commission. Horizon 2020 681137
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Microbiota ; HIV ; Vaccine ; T-cell ; IFNγ ; IL-22 ; Roseburia
Published in:	Vaccines (Basel), Vol. 11 (october 2023) , ISSN 2076-393X

DOI: 10.3390/vaccines11111663
PMID: 38005995

17 p, 1.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles