Post-Thaw Viable CD45+ Cells and Clonogenic Efficiency are Associated with Better Engraftment and Outcomes after Single Cord Blood Transplantation in Adult Patients with Malignant Diseases
Castillo, Nerea (Hospital Universitari Vall d'Hebron)
García Cadenas, Irene (Institut d'Investigació Biomèdica Sant Pau)
Barba, Pere (Hospital Universitari Vall d'Hebron)
Martino, Rodrigo (Institut d'Investigació Biomèdica Sant Pau)
Azqueta, Carmen (Banc de Sang i Teixits)
Ferrá, Christelle (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Canals, Carme (Banc de Sang i Teixits)
Sierra, Jorge (Institut d'Investigació Biomèdica Sant Pau)
Valcárcel, David (Hospital Universitari Vall d'Hebron)
Querol, Sergio (Banc de Sang i Teixits)
Universitat Autònoma de Barcelona. Departament de Medicina

Date:	2015
Abstract:	The quantity of cells is widely accepted as the main factor influencing the outcome after umbilical cord blood transplantation (UCBT) however, the quality of the cord blood units (CBUs) has been less studied. In order to determine the impact of qualitative variables in UCBT outcomes, we conducted a multicenter retrospective study in adult patients with hematological malignancies who underwent single UCBT after a common myeloablative conditioning regimen. One hundred and ten patients from 3 institutions [median age, 35 years (range 18-55)] were included. Quantitative (TNC and total CD34+cells) and qualitative variables [viable CD45+ (vCD45+), vCD34+ and clonogenic efficiency [(CLONE), quotient of post-thaw colony-forming units (CFU)] and pre-freeze CD34+ cells predicted engraftment in univariate analysis however, only 2 qualitative variables remained significant in the multivariate analysis. Infusion of more than 2 × 10 post-thaw vCD45+ cells per kilogram was significantly associated with faster neutrophil (P =. 01), platelet engraftment (P =. 01), higher disease-free (P =. 01) and overall survival (0. 02). In addition, CLONE ≥ 20% predicted a faster neutrophil (P =. 005), platelet engraftment (P =. 01) and contributed to decrease the non-relapse mortality (P =. 02). Our study suggests that the vCD45+ cells dose and CLONE are powerful surrogate markers of graft quality and can potentially help on CBUs selection if tested with representative reference samples.
Grants:	Fundació la Marató de TV3 20133230
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Quality ; Transplantation ; Umbilical cord blood
Published in:	Biology of blood and marrow transplantation, Vol. 21 Núm. 12 (december 2015) , p. 2167-2172, ISSN 1523-6536

DOI: 10.1016/j.bbmt.2015.08.016
PMID: 26319094

6 p, 529.7 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles