Liquid fructose supplementation in LDL-R-/- mice fed a western-type diet enhances lipid burden and atherosclerosis despite identical calorie consumption
Hutter, Natalia (Universitat de Barcelona)
Baena, Miguel (Universitat de Barcelona)
Sangüesa, Gemma (Universitat de Barcelona)
Dávalos, Alberto (Universidad Autónoma de Madrid)
Latasa, M.J. (Universidad Autónoma de Madrid)
Escolà-Gil, Joan Carles (Institut d'Investigació Biomèdica Sant Pau)
Sánchez, Rosa María (Universitat de Barcelona)
Roglans, Núria (Universitat de Barcelona)
Alegret, Marta (Universitat de Barcelona)
Laguna, Joan Carles (Universitat de Barcelona)
Universitat Autònoma de Barcelona

Data:	2015
Resum:	Background: Studies on humans have related the high consumption of fructose, especially in the form of sugar-sweetened beverages, to obesity, fatty liver, and hypercholesterolemia, all risk factors for atherosclerosis, and cardiovascular disease. We aimed to determine whether supplementation of liquid fructose (SLF), in either a normal, healthy chow or a Western-style chow, promoted the appearance of atherosclerosis in a rodent model. Methods: LDL receptor knockout mice were fed for twelve weeks with normal chow, normal chow plus ad libitum 15% fructose solution, Western chow, or Western chow plus ad libitum 15% fructose solution (W. +. F). Food and liquid intake and body weight were periodically monitored. At the end of the study, plasma and hepatic lipids, liver histology and expression of genes related to lipid handling were analyzed and histological and immunohistological analyses of atherosclerosis at the aortic origin was performed. Results: Total calorie intake was significantly increased in Western-fed vs normal chow-fed mice, but was not modified by SLF. SLF significantly increased body weight, visceral adiposity, plasma lipids and liver cholesterol content in Western-fed mice, probably due to an increase in de novo lipid synthesis. Aortic atherosclerotic total lesion area was significantly correlated to plasma lipids, being highest in W. +. F mice. Further, SLF induced higher immunostaining for macrophages and oxidized-LDL receptor, independently of lesion area and caloric burden. Conclusions: SLF, without changing total calorie intake, increases atherosclerosis, visceral adipose tissue and cholesterol burden in a background of overweight LDL receptor knockout mice consuming an unhealthy, Western-type solid rodent chow.
Ajuts:	Ministerio de Economía y Competitividad FISPI11/00315 Ministerio de Economía y Competitividad SAF2013-42982-R Generalitat de Catalunya SGR09-00413 Generalitat de Catalunya SGR13-00066
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	CVD ; Inflammation ; Lipid metabolism ; Macrophages ; NAFLD ; Sugar-sweetened beverages
Publicat a:	IJC Metabolic and Endocrine, Vol. 9 (january 2015) , p. 12-21, ISSN 2214-7624

DOI: 10.1016/j.ijcme.2015.10.002

10 p, 1.5 MB

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