Enhanced vascular permeability facilitates entry of plasma HDL and promotes macrophage-reverse cholesterol transport from skin in mice
Kareinen, Ilona (Wihuri Research Institute)
Cedó, Lídia (Institut d'Investigació Biomèdica Sant Pau)
Silvennoinen, Reija (Wihuri Research Institute (Finlàndia))
Laurila, Pirkka Perkka (National Institute for Health and Welfare (Finlàndia))
Jauhiainen, Matti (National Institute for Health and Welfare (Finlàndia))
Julve i Gil, Josep (Institut d'Investigació Biomèdica Sant Pau)
Blanco Vaca, Francisco (Institut d'Investigació Biomèdica Sant Pau)
Escolà-Gil, Joan Carles (Institut d'Investigació Biomèdica Sant Pau)
Kovanen, Petri T. (Wihuri Research Institute (Finlàndia))
Lee-Rueckert, Miriam (Wihuri Research Institute (Finlàndia))
Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular

Date:	2015
Abstract:	Reverse cholesterol transport (RCT) pathway from macrophage foam cells initiates when HDL particles cross the endothelium, enter the interstitial fluid, and induce cholesterol efflux from these cells. We injected [ 3 H] cholesterol-loaded J774 macrophages into the dorsal skin of mice and measured the transfer of macrophage-derived [ 3 H]cholesterol to feces [macrophage-RCT (m-RCT)]. Injection of histamine to the macrophage injection site increased locally vascular permeability, enhanced influx of intravenously administered HDL, and stimulated m-RCT from the histamine-treated site. The stimulatory effect of histamine on m-RCT was abolished by prior administration of histamine H1 receptor (H1R) antagonist pyrilamine, indicating that the histamine effect was H1R-dependent. Subcutaneous administration of two other vasoactive mediators, serotonin or bradykinin, and activation of skin mast cells to secrete histamine and other vasoactive compounds also stimulated m-RCT. None of the studied vasoactive mediators affected serum HDL levels or the cholesterol-releasing ability of J774 macrophages in culture, indicating that acceleration of m-RCT was solely due to increased availability of cholesterol acceptors in skin. We conclude that disruption of the endothelial barrier by vasoactive compounds enhances the passage of HDL into interstitial fluid and increases the rate of RCT from peripheral macrophage foam cells, which reveals a novel tissue cholesterol-regulating function of these compounds.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bradykinin ; Cholesterol ; Foam cells ; High density lipoprotein ; Histamine ; Lipoproteins ; Mast cells ; Serotonin
Published in:	Journal of Lipid Research, Vol. 56 Núm. 2 (january 2015) , p. 241-253, ISSN 1539-7262

DOI: 10.1194/jlr.M050948
PMID: 25473102

13 p, 900.7 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
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