Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation
Lister, Nicholas (University of New South Wales Sydney)
Milton, Ashley M. (University of New South Wales Sydney)
Patel, Hardip R. (Australian National University)
Waters, Shafagh A. (University of New South Wales)
Hanrahan, Benjamin J. (University of New South Wales Sydney)
McIntyre, Kim L. (University of New South Wales Sydney)
Livernois, Alexandra M. (Zymeworks)
Horspool, William B. (University of New South Wales Sydney)
Wee, Lee Kian (University of New South Wales Sydney)
Ringel, Alessa R. (Institute of Chemistry and Biochemistry. Freie Universität Berlin)
Mundlos, Stefan (Charité-Universitätsmedizin Berlin)
Robson, Michael I. (University of Edinburgh)
Shearwin-Whyatt, Linda (University of Adelaide)
Grutzner, Frank (School of Biological Sciences. University of Adelaide)
Marshall Graves, Jennifer A. (Institute of Applied Ecology. University of Canberra)
Ruiz-Herrera Moreno, Aurora (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Waters, Paul D. (School of Biotechnology and Biomolecular Sciences. Faculty of Science. University of New South Wales Sydney)
Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí"

Date:	2024
Abstract:	Heteromorphic sex chromosomes (XY or ZW) present problems of gene dosage imbalance between sexes and with autosomes. A need for dosage compensation has long been thought to be critical in vertebrates. However, this was questioned by findings of unequal mRNA abundance measurements in monotreme mammals and birds. Here, we demonstrate unbalanced mRNA levels of X genes in platypus males and females and a correlation with differential loading of histone modifications. We also observed unbalanced transcripts of Z genes in chicken. Surprisingly, however, we found that protein abundance ratios were 1:1 between the sexes in both species, indicating a post-transcriptional layer of dosage compensation. We conclude that sex chromosome output is maintained in chicken and platypus (and perhaps many other non therian vertebrates) via a combination of transcriptional and post-transcriptional control, consistent with a critical importance of sex chromosome dosage compensation.
Grants:	Agencia Estatal de Investigación PID2020-112557GB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00122
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Proceedings of the National Academy of Sciences of the United States of America, Vol. 121, Issue 32 (July 2024) , art. e2322360121, ISSN 1091-6490

DOI: 10.1073/pnas.2322360121
PMID: 39074288

7 p, 5.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
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