Kinetics and toxicity of nanoplastics in ex vivo exposed human whole blood as a model to understand their impact on human health
Arribas Arranz, Jéssica (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villacorta, Aliro (Universidad Arturo Prat. Facultad de Recursos Naturales Renovables)
Rubio Lorente, Laura (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
García-Rodríguez, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Sánchez, Gloria (Consejo Superior de Investigaciones Científicas (Espanya))
Llorca, Marta (Consejo Superior de Investigaciones Científicas (Espanya))
Farre, Marinella (Consejo Superior de Investigaciones Científicas (Espanya))
Ferrer-Crespo, Juan Francisco (AIMPLAS Instituto Tecnológico del Plástico)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2024
Descripció:	13 pàg.
Resum:	The ubiquitous presence of nanoplastics (NPLs) in the environment is considered of great health concern. Due to their size, NPLs can cross both the intestinal and pulmonary barriers and, consequently, their presence in the blood compartment is expected. Understanding the interactions between NPLs and human blood components is required. In this study, to simulate more adequate real exposure conditions, the whole blood of healthy donors was exposed to five different NPLs: three polystyrene NPLs of approximately 50 nm (aminated PS-NH2, carboxylated PS-COOH, and pristine PS- forms), together with two true-to-life NPLs from polyethylene terephthalate (PET) and polylactic acid (PLA) of about 150 nm. Internalization was determined in white blood cells (WBCs) by confocal microscopy, once the different main cell subtypes (monocytes, polymorphonucleated cells, and lymphocytes) were sorted by flow cytometry. Intracellular reactive oxygen species (iROS) induction was determined in WBCs and cytokine release in plasma. In addition, hemolysis, coagulation, and platelet activation were also determined. Results showed a differential uptake between WBC subtypes, with monocytes showing a higher internalization. Regarding iROS, lymphocytes were those with higher levels, which was observed for different NPLs. Changes in cytokine release were also detected, with higher effects observed after PLA- and PS-NH2-NPL exposure. Hemolysis induction was observed after PS- and PS-COOH-NPL exposure, but no effects on platelet functionality were observed after any of the treatments. To our knowledge, this is the first study comprehensively evaluating the bloodstream kinetics and toxicity of NPL from different polymeric types on human whole blood, considering the role played by the cell subtype and the NPLs physicochemical characteristics in the effects observed after the exposures.
Ajuts:	European Commission 801370 European Commission 965196 Agencia Estatal de Investigación PID2020-116789 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Hemolysis ; Nanoplastics ; Oxidative stress ; Platelets functionality ; Uptake ; SDG 3 - Good Health and Well-being
Publicat a:	Science of the total environment, Vol. 948 (October 2024) , ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2024.174725
PMID: 39009158

13 p, 2.6 MB

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