Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
Rossi-Herring, Guido (Universidad del Valle de Guatemala (UVG))
Belmonte, Thalia (Instituto de Salud Carlos III)
Rivas-Urbina, Andrea (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Benitez, Sonia (Institut d'Investigació Biomèdica Sant Pau)
Rotllan, Noemi (Institut d'Investigació Biomèdica Sant Pau)
Crespo García, Javier (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Llorente-Cortés, Vicenta (Institut d'Investigació Biomèdica Sant Pau)
Sanchez-Quesada, Jose Luis (Institut d'Investigació Biomèdica Sant Pau)
Gonzalo Calvo, David de (Instituto de Salud Carlos III)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	Lipoproteins have been described as microRNAs (miRNAs) carriers. Unfortunately, the bibliography on this topic is scarce and shows a high variability between independent investigations. In addition, the miRNA profiles of the LDL and VLDL fractions have not been completely elucidated. Here, we profiled the human circulating lipoprotein-carried miRNome. Lipoprotein fractions (VLDL, LDL and HDL) were isolated from the serum of healthy subjects by ultracentrifugation and purified by size-exclusion chromatography. A panel of 179 miRNAs commonly expressed in circulation was evaluated in the lipoprotein fractions using quantitative real-time PCR (qPCR) assays. A total of 14, 4 and 24 miRNAs were stably detected in the VLDL, LDL and HDL fractions, respectively. VLDL- and HDL-miRNA signatures were highly correlated (rho 0. 814), and miR-16-5p, miR-142-3p, miR-223-3p and miR-451a were among the top 5 expressed miRNAs in both fractions. miR-125a-5p, miR-335-3p and miR-1260a, were detected in all lipoprotein fractions. miR-107 and miR-221-3p were uniquely detected in the VLDL fraction. HDL showed the larger number of specifically detected miRNAs (n = 13). Enrichment in specific miRNA families and genomic clusters was observed for HDL-miRNAs. Two sequence motifs were also detected for this group of miRNAs. Functional enrichment analysis including the miRNA signatures from each lipoprotein fraction suggested a potential role in mechanistic pathways previously associated with cardiovascular disease: fibrosis, senescence, inflammation, immune response, angiogenesis, and cardiomyopathy. Collectively, our results not only support the role of lipoproteins as circulating miRNA carriers but also describe for the first time the role of VLDL as a miRNA transporter.
Ajuts:	Ministerio de Economía y Competitividad PI16/00471 Instituto de Salud Carlos III PI20/00334 Instituto de Salud Carlos III PI20/00577 Instituto de Salud Carlos III PI21/01523 Ministerio de Ciencia e Innovación CB07/06/2008 Ministerio de Economía y Competitividad CB16/11/00276
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	HDL ; LDL ; Lipoprotein ; MicroRNA ; VLDL
Publicat a:	Biomedicine & pharmacotherapy, Vol. 162 (june 2023) , p. 114623, ISSN 1950-6007

DOI: 10.1016/j.biopha.2023.114623
PMID: 37023624

9 p, 2.1 MB

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