Multimodal HOCl-responsive MEH-PPV nanoparticles for anti-inflammatory imaging and therapy
Villar-Alvarez, Eva (Institut Català de Nanociència i Nanotecnologia)
Parron-Onate, Sara (Institut Català de Nanociència i Nanotecnologia)
Wienskowska, Olga (Institut Català de Nanociència i Nanotecnologia)
Carrascull-Marín, Aleix (Institut Català de Nanociència i Nanotecnologia)
Bellacanzone, Christian (Institut Català de Nanociència i Nanotecnologia)
Lorenzo Rivera, Julia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Ruiz-Molina, Daniel (Institut Català de Nanociència i Nanotecnologia)
Roscini, Claudio (Institut Català de Nanociència i Nanotecnologia)
Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí"

Date:	2024
Abstract:	Inflammatory disorders often correlate with an unusually high intracellular production of hypochlorous acid (HOCl). Therefore, its rapid, sensitive, and specific detection is crucial for an early diagnosis and treatment evaluation. While nanoparticles for detection have already been reported, multimodal nanoparticles that simultaneously detect and eliminate reactive oxygen species (including the excess of HOCl) are scarce despite their interest. Herein, we developed highly selective fluorescent nanoparticles using the copolymer poly(2-methoxy-5-(2-ethylhexyloxy)-1, 4-phenylenevinylene) (MEH-PPV), with (MEH@CS) and without a chitosan coating (MEH). The conjugated polymer is oxidized in the presence of HOCl, exhibiting a rapid (in less than 30 s) and sensitive fluorescence turn-off response with a log-log linear HOCl relationship within dynamic ranges of ≈ 0. 784-83 μM and ≈ 0. 384-55 μM for MEH and MEH@CS NPs, respectively, allowing to monitor basal HOCl levels within the standard physiological concentration range (5 - 25 μM) and its differentiation from overproduction. Moreover, the nanoparticles can encapsulate and release quercetin (a powerful natural scavenger for HOCl), leading not only to monitoring but also to a reduction in pro-inflammatory cytokines of inflammation-stimulated macrophage cells.
Grants:	Agencia Estatal de Investigación FJC2018-036356-I Agencia Estatal de Investigación PID2021-127983OB-C21 Agencia Estatal de Investigación PID2021-127983OB-C22 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00135 Agencia Estatal de Investigación CEX2021-001214-S
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Fluorescence probe ; Hypochlorous acid ; Inflammatory disease ; MEH-PPV QuercetinTheranostic
Published in:	Sensors and Actuators B: Chemical, Vol. 417 (October 2024) , art. 136150, ISSN 0925-4005

DOI: 10.1016/j.snb.2024.136150

15 p, 8.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles