Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy
Durán Fernández-Feijóo, Cristina (Hospital Álvaro Cunqueiro (Vigo))
Rodríguez-Fanjul, Javier (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lopez-Abat, Miriam (Hospital Clínic i Provincial de Barcelona)
Hadley, Stephanie (Vanderbilt University School of Medicine)
Cavia, Mónica (Universidad de Burgos)
Muñiz, Pilar (Universidad de Burgos)
Arnaez, Juan (Hospital Universitario de Burgos)
Fernández-Lorenzo, José Ramón (Hospital Álvaro Cunqueiro (Vigo))
Camprubí Camprubí, Marta (Hospital Clínic i Provincial de Barcelona)

Date:	2021
Abstract:	Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated, hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia + allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio and carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and cerebrospinal fluid, and 8-iso-prostaglandin F2α was measured in brain tissue. Plasma 2,2'-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection in HIE.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Allopurinol ; Hypothermia ; Hypoxic-ischemic encephalopathy ; Oxidative stress ; Oxidative damage
Published in:	Antioxidants, Vol. 10, Núm. 10 (September 2021) , ISSN 2076-3921

DOI: 10.3390/antiox10101523
PMID: 34679658

11 p, 1.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles