Genome-wide transcriptional analysis of T cell activation reveals differential gene expression associated with psoriasis
Palau, Núria (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Julià Cano, Antonio (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ferrándiz, Carlos (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Puig Sanz, Lluís (Institut d'Investigació Biomèdica Sant Pau)
Fonseca, Eduardo (Hospital Abente y Lago)
Fernández, Emilia (Hospital Universitario de Salamanca)
López Lasanta, María (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Tortosa, Raül (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Marsal, Sara (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona. Departament de Medicina

Fecha:	2013
Resumen:	Background: Psoriasis is a chronic autoimmune disease in which T cells have a predominant role in initiating and perpetuating the chronic inflammation in skin. However, the mechanisms that regulate T cell activation in psoriasis are still incompletely understood. The objective of the present study was to characterize the main genetic pathways associated with T cell activation in psoriasis. Results: Gene expression profiles from in vitro activated T cells were obtained from 17 psoriasis patients and 7 healthy controls using Illumina HT-12 v4 microarrays. From a total of 47,321 analyzed transcripts, 42 genes were found to be differentially expressed between psoriasis and controls (FDR p-value < 0. 1, absolute fold-change > 1. 2). Using an independent cohort of 8 patients and 8 healthy controls we validated the overexpression of SPATS2L (p-value =0. 0009) and KLF6 (p-value =0. 0012) genes in activated T cells from psoriasis patients. Using weighted correlation analysis we identified SPATS2L and KLF6 coexpression networks, which were also significantly associated with psoriasis (p-value < 0. 05). Gene Ontology analysis allowed the identification of several biological processes associated with each coexpression network. Finally, using Gene Set Enrichment Analysis over the global T cell transcriptome we also found additional genetic pathways strongly associated with psoriasis (p-value < 0. 0001). Conclusions: This study has identified two new genes, SPATS2L and KLF6, strongly associated with T cell activation in psoriasis. Functional analyses of the gene expression profiles also revealed new biological processes and genetic pathways associated with psoriasis. The results of this study provide an important insight into the biology of this common chronic inflammatory disease. © 2013 Palau et al. ; licensee BioMed Central Ltd.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Gene expression ; Gene network ; Genetic pathway ; Microarray ; Psoriasis ; T cell
Publicado en:	BMC genomics, Vol. 14 Núm. 1 (25 2013) , p. 825, ISSN 1471-2164

DOI: 10.1186/1471-2164-14-825
PMID: 24267790

13 p, 547.4 KB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut de Recerca Sant Pau
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