Development and validation of a minimally invasive and image-guided tape stripping method to sample atopic skin in children
Yélamos, Oriol (Institut d'Investigació Biomèdica Sant Pau)
Andersen, D. (Technical University of Denmark)
Pont, M. (Almirall R&D)
Iglesias, P. (Hospital Clínic i Provincial de Barcelona)
Potrony, Miriam (Centro de Investigación Biomédica en Red de Enfermedades Raras)
Domínguez, M. (Hospital Clínic i Provincial de Barcelona)
Herrero, A. (Hospital Clínic i Provincial de Barcelona)
Alejo, Brien (Hospital Clínic i Provincial de Barcelona)
Mateu, J. (Hospital Clínic i Provincial de Barcelona)
Røpke, M. (LeoPharma A/S)
Danneskiold-Samsøe, N.B. (University of Copenhagen)
Malvehy, J. (Centro de Investigación Biomédica en Red de Enfermedades Raras)
Guy, R.H. (University of Bath)
Brix, S. (Technical University of Denmark)
Puig, Susana (Centro de Investigación Biomédica en Red de Enfermedades Raras)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	Molecular skin profiling techniques, typically performed on skin samples taken by punch biopsy, have enhanced the understanding of the pathophysiology of atopic dermatitis (AD), thereby enabling the development of novel targeted therapeutics. However, punch biopsies are not always feasible or desirable, and novel minimally invasive methods such as skin tape stripping have been developed. To develop, optimize and validate a novel tape stripping method guided by noninvasive in vivo skin imaging to sample atopic skin in children. Skin tape stripping-based procedures were compared and optimized using data from 30 healthy controls (HCs: 5 adults, 25 children) and 39 atopic children. Evaluations were guided by high-resolution photography, reflectance confocal microscopy, optical coherence tomography and transepidermal water loss measurements. We assessed and compared adverse events (AEs), the time needed to perform the sampling and the cDNA levels obtained from the tapes. Tape stripping methods based on previously described protocols resulted in erosions in all participants and required a median time of 65min to perform (range 60-70min), but provided good cDNA yield. Shorter durations appeared less invasive but provided lower cDNA yield. The final optimized tape stripping protocol, using 11 tapes of 22mm in diameter, each applied twice for 5s with 90° rotation, did not produce significant AEs, was completed within a median time of 7min (range 5-15min) and provided good cDNA yield both in HCs and atopic children. Our minimally invasive method is safe and reliable, and provides reproducible acquisition of cDNA in atopic children. In addition, it enables rapid sample collection, a crucial factor in clinical practice.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article de revisió ; recerca ; Versió publicada
Matèria:	Adult ; Biopsy ; Child ; Dermatitis, Atopic ; DNA, Complementary ; Epidermis ; Humans ; Skin ; Specimen Handling
Publicat a:	Clinical and Experimental Dermatology, Vol. 48 Núm. 2 (february 2023) , p. 80-88, ISSN 1365-2230

DOI: 10.1093/ced/llac040
PMID: 36730521

9 p, 2.1 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut de Recerca Sant Pau
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