Type 2 Diabetes in Obesity : A Systems Biology Study on Serum and Adipose Tissue Proteomic Profiles
Arderiu, Gemma (Institut d'Investigació Biomèdica Sant Pau)
Mendieta, Guiomar (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Gallinat, Alex (Institut d'Investigació Biomèdica Sant Pau)
Lambert, Carmen (IPSA-Instituto de Investigación Sanitaria del Principado de Asturias)
Díez-Caballero, Alberto (Centro Médico Teknon. Grupo Quiron Salut)
Ballesta, Carlos (Centro Médico Teknon. Grupo Quiron Salut)
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	Obesity is associated with metabolic disorders such as insulin resistance and type 2 diabetes mellitus (T2DM), further increasing an already heightened cardiovascular risk. Here, amongst obese class III bariatric surgery patients, we have investigated the effect of T2DM in serum and in two, same patient, adipose tissue (AT) depots through proteomic profile expression analyses. Serum and AT samples from subcutaneous (SAT) and visceral (VAT) fat were collected during bariatric surgery. Bead-based targeted multiplex assay systems were used to simultaneously detect and quantify multiple targets in serum samples (targeted proteomics) and analyze changes in adipokine serum composition. AT samples were assessed through an untargeted proteomics approach. Through a systems biology analysis of the proteomic data, information on the affected biological pathways was acquired. In obese class III individuals, the presence of T2DM induced a significantly higher systemic release of ghrelin, GLP-1, glucagon, MMP3, BAFF, chitinase 3-like 1, TNF-R1 and TNF-R2, and a lower systemic release of IL-8. SAT and VAT proteomes belonging to the same patient showed significant differences in local protein content. While the proteins upregulated in VAT were indicative of metabolic dysregulation, SAT protein upregulation suggested adequate endocrine regulation. The presence of T2DM significantly affected VAT protein composition through the upregulation of dysregulating metabolic pathways, but SAT protein composition was not significantly modified. Our results show that T2DM induces metabolic dysregulation in obese individuals with changes in systemic marker levels and impairment of proteostasis in VAT but not in SAT.
Ajuts:	Instituto de Salud Carlos III PI20/01517 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1480
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Diabetes ; Proteomic analysis ; Subcutaneous adipose tissue ; Visceral adipose tissue
Publicat a:	International journal of molecular sciences, Vol. 24 Núm. 1 (january 2023) , p. 827, ISSN 1422-0067

DOI: 10.3390/ijms24010827
PMID: 36614270

20 p, 21.7 MB

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