Methodological Assessment of ExoGAG for Isolation of Cerebrospinal Fluid Extracellular Vesicles as a Source of Biomarkers
Salvat-Rovira, Nil (Institut de Recerca Sant Pau)
Vazquez-Oliver, Anna (Institut de Recerca Sant Pau)
Rivas Asensio, Elisa (Institut de Recerca Sant Pau)
Herrero-Lorenzo, Marina (Universitat de Barcelona)
Gámez-Valero, Ana (Universitat de Barcelona)
Pérez-Pérez, Jesús (Institut de Recerca Sant Pau)
Izquierdo, Cristina (Institut de Recerca Sant Pau)
Campolongo, Antonia (Institut de Recerca Sant Pau)
Martí, Eulàlia (Universitat de Barcelona)
Kulisevsky, Jaime (Institut de Recerca Sant Pau)
Pérez-González, Rocío (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)

Date:	2024
Abstract:	Extracellular vesicles (EVs) in cerebrospinal fluid (CSF) represent a valuable source of biomarkers for central nervous system (CNS) diseases, offering new pathways for diagnosis and monitoring. However, existing methods for isolating EVs from CSF often prove to be labor-intensive and reliant on specialized equipment, hindering their clinical application. In this study, we present a novel, clinically compatible method for isolating EVs from CSF. We optimized the use of ExoGAG, a commercially available reagent that has been tested in plasma, urine and semen, and compared it directly with differential ultracentrifugation using Western blotting, protein quantification, nanoparticle tracking analysis, and cryogenic electron microscopy. Additionally, we analyzed the presence of specific microRNAs (miRNAs) known to be present in CSF-derived EVs. Our data demonstrate that ExoGAG is an effective method for isolating EVs from CSF, yielding a higher amount of EVs compared to traditional ultracentrifugation methods, and with comparable levels of specific miRNAs. In conclusion, the use of ExoGAG in a clinical setting may facilitate the testing of biomarkers essential for tracking brain pathology in CNS diseases.
Grants:	Agencia Estatal de Investigación PID2022-137426OA-I00 Instituto de Salud Carlos III PI17/001885 Instituto de Salud Carlos III CP21/00009
Note:	Altres ajuts: Centro de Investigación Biomédica en Red Enfermedades Neurodegenerativas (CB06/05/0047)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Extracellular vesicles ; Cerebrospinal fluid ; Isolation method ; Ultracentrifugation ; ExoGAG ; Biomarkers ; Mirnas
Published in:	International journal of molecular sciences, Vol. 25, Núm. 24 (december 2024) , ISSN 1422-0067

DOI: 10.3390/ijms252413705
PMID: 39769471

13 p, 1.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles