Cellular strategies against metal exposure and metal localization patterns linked to phosphorus pathways in Ochrobactrum anthropi DE2010
Villagrasa, Eduard (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Palet i Ballús, Cristina (Universitat Autònoma de Barcelona. Departament de Química)
López-Gómez, Irene (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Gutiérrez, Diana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Esteve, Isabel (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Sánchez Chardi, Alejandro (Universitat de Barcelona. Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals)
Solé Cornellà, Antoni (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Universitat Autònoma de Barcelona. Servei de Microscòpia

Date:	2021
Abstract:	Cytotoxic, chemical, biochemical, compositional, and morphometric responses were analyzed against heavy metal exposure in Ochrobactrum anthropi DE2010, an heterotrophic bacterium isolated from Ebro Delta microbial mats (Tarragona, NE Spain). Several parameters of effect and exposure were evaluated to determine tolerance to a range of cadmium (Cd), lead (Pb(II)), copper (Cu(II)), chromium (Cr(III)), and zinc (Zn) concentrations. Additionally, removal efficiency, polyphosphate production and metal localization patterns were also analyzed. O. anthropi DE2010 showed high resistance to the tested metals, supporting concentrations of up to 20 mM for Zn and 10 mM for the rest of the elements. The bacterium also demonstrated a high removal capacity of metals-up to 90 % and 40 % for Pb(II) and Cr(III), respectively. Moreover, polyphosphate production was strongly correlated with heavy metal concentration, and three clear cell localization patterns of metals were evidenced using compositional and imaging techniques: (i) extracellular in polyphosphate granules for Cu(II); (ii) in periplasmic space forming crystals with phosphorus for Pb(II); and (iii) intracytoplasmic in polyphosphate inclusions for Pb(II), Cr(III), and Zn. The high resistance and metal sequestration capacity of O. anthropi DE2010 both highlight its great potential for bioremediation strategies, especially in Pb and Cr polluted areas.
Grants:	Ministerio de Economía y Competitividad CTQ2014-54553-C3-2-R Ministerio de Ciencia e Innovación CGL2008-01891
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Bioaccumulation ; Biomineralization ; Heavy metal ; Polyphosphate production ; Sequestration
Published in:	Journal of hazardous materials, Vol. 402 (January 2021) , art. 123808, ISSN 1873-3336

DOI: 10.1016/j.jhazmat.2020.123808
PMID: 33254804

Postprint 27 p, 347.2 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles