Characterization of Immune Cell Populations and Acid-Sensing Receptors in the Human Esophagus
Fortea, Marina (Hospital Universitari Vall d'Hebron)
Hacour, Leen (Katholieke Universiteit te Leuven (1970-))
Sancho, Francesc (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Boada, Carlos (Hospital Universitari Vall d'Hebron)
Sevillano-Aguilera, Cesar (Hospital Universitari Vall d'Hebron)
González Castro, Ana Maria (Hospital Universitari Vall d'Hebron)
Salvo-Romero, Eloisa (Hospital Universitari Vall d'Hebron)
Lobo Álvarez, Beatriz (Hospital Universitari Vall d'Hebron)
Guagnozzi, Danila (Hospital Universitari Vall d'Hebron)
Ceulemans, Laurens J. (Katholieke Universiteit te Leuven (1970-))
Pirenne, Jacques (Katholieke Universiteit te Leuven (1970-))
Alonso Cotoner, Carmen (Hospital Universitari Vall d'Hebron)
Santos, Javier (Hospital Universitari Vall d'Hebron)
Farré, Ricard (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Vicario Perez, Maria (Hospital Universitari Vall d'Hebron)
Universitat Autònoma de Barcelona

Data:	2024
Resum:	Introduction: Esophageal inflammatory diseases are frequent diagnoses in clinical practice and have diverse etiologies, the most common being those associated with the exposure to gastric content, drugs and allergens. In diseases, the immunological component is well identified in endoscopic biopsies, which mainly contain the epithelium and the lamina propria; however, deeper layers are less studied. Moreover, the esophageal capacity of sensing luminal compounds is poorly understood. Methods: In transmural sections from proximal, middle and distal esophagus obtained from deceased patients, we performed a phenotypic analysis of the main immune cell populations and acid-sensing receptors by immunohistochemistry and immunofluorescence methods. Results: A total of nine donors were studied (absence of pathology, optimal tissue preservation and orientation). We found the following: (1) the vascular papillae and the lamina propria are the most infiltrated layers by the lymphoid lineage (T and B lymphocytes), followed by the epithelium, while the smooth muscular layers are mainly populated by the myeloid lineage (macrophages and mast cells); (2) intraepithelial macrophages are consistently found along the esophagus; and (3) eosinophils are absent in all the esophageal layers. The acid-sensing receptors ASIC-1, ASIC-2 and δENAC are expressed in the esophageal epithelium and in the lamina propria, yet only ASIC-2 is expressed in the muscularis mucosae. Conclusions: The human esophagus contains a differential distribution of immune cells and acid-sensing receptors across its layers. This study extends the esophageal histological knowledge previously described and reinforces its role as a defensive and sensing organ.
Ajuts:	Ministerio de Economía y Competitividad PI16/00583 Instituto de Salud Carlos III PI19/01643 Ministerio de Economía y Competitividad CPII16/00031 Ministerio de Ciencia e Innovación CM08/00229 Ministerio de Economía y Competitividad FI12/00254 Ministerio de Economía y Competitividad PI15/00301
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Esophagus ; Immune system ; Acid-sensing receptors
Publicat a:	Gastroenterology Insights, Vol. 15 Núm. 3 (september 2024) , p. 819-834, ISSN 2036-7422

DOI: 10.3390/gastroent15030058

16 p, 4.7 MB

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