Evaluation of blood cytotoxicity against tumor cells using a live-cell imaging platform
Salvia, Roser (Institut Germans Trias i Pujol)
G. Rico, Laura (Institut Germans Trias i Pujol)
Morán, Teresa (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Olszowy, Michael W. (Sartorius Stedim North America, inc., Arvada, Colorado, USA)
Ward, Michael D. (Thermo Fisher Scientific, Fort Collins, Colorado, USA)
Petriz, Jordi (Institut Germans Trias i Pujol)
Universitat Autònoma de Barcelona. Departament de Medicina

Date:	2025
Abstract:	Cellular cytotoxicity is an important mechanism of the immune system to clear infections and eliminate tumor cells. Its two main mediators are cytotoxic T lymphocytes and natural killer (NK) cells. In lung cancer, intratumoral NK cells show reduced cytolytic potential and one third of patients do not express HLA-I proteins, which activate NK cells in a process termed "absent self-recognition". In this work, we investigate NK cytotoxicity as a potential oncological biomarker that informs patient status and predicts response to treatment. We describe a simple and rapid test to analyze NK cytotoxicity without the need for large volumes of blood, requiring short processing time and reduced use of both reagents and blood samples, using the IncuCyte live imaging technique.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00002
Rights:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Blood cytotoxicity ; Live-cell imaging ; Cancer
Published in:	Cytometry. Part A, 2025 , ISSN 1552-4930

DOI: 10.1002/cyto.a.24930

Available from: 2099-01-01 Postprint

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles