In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant Escherichia coli
Cuyàs, Berta (Universitat Autònoma de Barcelona)
Cantó, Elisabet (Institut de Recerca Sant Pau)
Sanchez-Ardid, Elisabet (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Miró, Elisenda (Institut de Recerca Sant Pau)
Alvarado-Tapias, Edilmar (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Román, Eva (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Poca Sans, Maria (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Navarro Risueño, Ferran (Institut de Recerca Sant Pau)
Ferrero-Gregori, Andreu (Institut de Recerca Sant Pau)
Escorsell, Àngels (Universitat Autònoma de Barcelona. Departament de Medicina)
Vidal, Silvia (Institut de Recerca Sant Pau)
Soriano, German (Universitat Autònoma de Barcelona. Departament de Medicina)
Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia

Date:	2025
Abstract:	Infections caused by multidrug-resistant organisms (MDRO) are linked to poor outcomes, particularly in patients with cirrhosis. The underlying mechanisms are not fully understood and may involve a different immune response against MDRO. This study aimed to compare the in vitro immune response between multidrug-resistant (MDR) Escherichia coli and antibiotic-susceptible E. coli strains. Surface protein extract and DNA extract were obtained from MDR E. coli (n = 6) and antibiotic-susceptible E. coli (n = 6) strains isolated from infected patients with cirrhosis. The extracts were used to stimulate in vitro peripheral blood mononuclear cells from healthy donors. After 48 h, cytokine levels (IFN-γ, IL-1β, IL-10, IL-12p70, MCP-1, IL-8, IL-6, MIP-1α, and MIP-1β) were measured. We observed no significant differences in cytokine production between MDR and susceptible strains. However, we identified notable interindividual variability in cytokine production for most of the cytokines studied. Only IFN-γ and IL-6 in surface extract and MCP-1 in DNA extract showed similar levels across all donors. We conclude that the cytokine profiles induced by MDR E. coli in vitro were similar to those in susceptible strains. These findings suggest that the poor prognosis associated with MDR E. coli infections is not due to a differential immune response but rather to other factors.
Grants:	Instituto de Salud Carlos III PI21/01995
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Resistance ; Cirrhosis ; Immune response ; MDRO ; Escherichia coli ; Cytokines
Published in:	Microorganisms, Vol. 13 Núm. 5 (May 2025) , ISSN 2076-2607

DOI: 10.3390/microorganisms13051164
PMID: 40431335

16 p, 4.9 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles