Advancing our understanding of root development : Technologies and insights from diverse studies
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Moreno-Risueno, Miguel Angel (Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas)
Strader, Lucia C. (Duke University. Department of Biology)
Goodnight, Alexandra V. (North Carolina State University. N.C. Plant Sciences Initiative)
Sozzani, Rosangela (North Carolina State University. Department of Plant and Microbial Biology)

Títol variant:	Advancing root biology : Technologies and insights from diverse examples
Data:	2025
Resum:	Understanding root development is critical for enhancing plant growth and health, and advanced technologies are essential for unraveling the complexities of these processes. In this review, we highlight select technological innovations in the study of root development, with a focus on the transformative impact of single-cell gene expression analysis. We provide a high-level overview of recent advancements, illustrating how single-cell RNA sequencing (scRNA-seq) has become a pivotal tool in plant biology. scRNA-seq has revolutionized root biology by enabling detailed, cell-specific analysis of gene expression. This has allowed researchers to create comprehensive root atlases, predict cell development, and map gene regulatory networks (GRNs) with unprecedented precision. Complementary technologies, such as multimodal profiling and bioinformatics, further enrich our understanding of cellular dynamics and gene interactions. Innovations in imaging and modeling, combined with genetic tools like CRISPR, continue to deepen our knowledge of root formation and function. Moreover, the integration of these technologies with advanced biosensors and microfluidic devices has advanced our ability to study plant-microbe interactions and phytohormone signaling at high resolution. These tools collectively provide a more comprehensive understanding of root system architecture and its regulation by environmental factors. As these technologies evolve, they promise to drive further breakthroughs in plant science, with substantial implications for agriculture and sustainability.
Nota:	Altres ajuts: grants "Estancias de personal docente y/o investigador senior en centros extranjeros 2022" (PRX22-00348 and PRX22/00429, respectively) sponsored by the Fulbright Program and the Ministerio de Universidades (MUNI).
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Llengua:	Anglès
Document:	Article de revisió ; recerca ; Versió acceptada per publicar
Matèria:	Gene Regulatory Networks ; Plant Development ; Plant Roots ; Sequence Analysis ; RNA ; Single-Cell Analysis
Publicat a:	Plant physiology, Vol. 197, Issue 2 (February 2025) , art. kiae605, ISSN 1532-2548

DOI: 10.1093/plphys/kiae605

Disponible a partir de: 2026-02-28 Postprint

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