Resistance to SARS-CoV-2 infection in camelid nasal organoids is associated with lack of ACE2 expression
Breugem, Tim (Erasmus Medical Center. Viroscience Department)
Riesebosch, Samra (Amsterdam University Medical Center)
Schipper, Debby (Erasmus Medical Center. Viroscience Department)
Mykytyn, Anna Z. (Erasmus Medical Center. Viroscience Department)
van den Doel, Petra (Erasmus Medical Center. Viroscience Department)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Lamers, Mart M. (Duke-NUS Medical School)
Haagmans, Bart L. (Erasmus Medical Center. Viroscience Department)

Date:	2024
Abstract:	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects a variety of animal species. Susceptibility to SARS-CoV-2 is primarily determined by the utilization of the viral receptor, ACE2. SARS-CoV-2 can utilize a broad range of animal ACE2 isoforms in vitro, including the ACE2 from various camelid species. However, experimental infection of these animals does not lead to productive infection or seroconversion. In this study, we investigate the susceptibility of camelids to SARS-CoV-2 using novel well-differentiated camelid nasal organoids. We show that camelid nasal organoids are highly susceptible to Middle East respiratory syndrome coronavirus (MERS-CoV) infection, but not to infection with different SARS-CoV-2 variants (614G, BA. 1 or EG. 5. 1. 1). All viruses efficiently infected human airway organoids. Immunohistochemistry analysis revealed the absence of ACE2 on camelid nasal organoids and dromedary camel upper respiratory tract. In contrast, DPP4 was expressed in both camelid nasal organoids and the camel upper respiratory tract, which correlates with MERS-CoV infection. This study indicates that the camelid upper respiratory tract lacks expression of ACE2, which is associated with resistance to SARS-CoV-2 infection.
Note:	Altres ajuts: ICRAD (Project "ConVErgence") GA n°862605
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Restriction factors ; SARS-CoV-2 ; Viral reservoirs ; Virus-host interactions
Published in:	npj Viruses, Vol. 2 (september 2024) , ISSN 2948-1767

DOI: 10.1038/s44298-024-00054-0
PMID: 40295820

8 p, 4.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles