Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
Toledo-Rubio, Verónica (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Vázquez Gómez, Esther, dir. (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Platas, Gemma (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Domingo-Espin, Joan (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Steinkamp, Elke (Bernhard-Nocht-Institut für Tropenmedizin)
Garcia-Fruitos, Elena (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2010
Abstract:	Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. Usually, the formation of soluble aggregates remains unperceived because analytical methods such as dynamic light scattering are not routinely applied as quality control procedures. The authors have developed a methodologically simple and fast procedure, based on microdialysis and image processing, that reveals the aggregation tendency of a given protein in a specific environment. Because they also show a good correlation between macroscopic aggregation and soluble aggregate formation, the microdialysis approach also permits an estimation of the occurrence of soluble aggregates.
Grants:	Ministerio de Economía y Competitividad BI02007-61194 Ministerio de Economía y Competitividad EUI2008-03610 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-108
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Protein aggregation ; Nanoparticles ; Dynamic light scattering ; Proto-aggregates ; Soluble aggregates
Published in:	Journal of biomolecular screening, Vol. 15, No. 4 (April 2010) , p. 453-457, ISSN 1552-454X

DOI: 10.1177/1087057110363911
PMID: 20233904

Post-print 11 p, 58.8 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles