The angiogenic gene profile of circulating endothelial progenitor cells from ischemic stroke patients
Navarro Sobrino, Míriam (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Hernández-Guillamon, Mar (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Fernandez-Cadenas, Israel (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ribo, Marc (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Romero, Ignacio A. (Department of Life Sciences, The Open University, Milton Keynes, UK)
Couraud, Pierre-Olivier (Institut Cochin (França). Division of Hematology and Medical Oncology)
Weksler, Babette Barbash (Weill Medical College of Cornell University, New York, NY, USA)
Montaner, Joan (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Rosell Novel, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Data:	2013
Resum:	The identification of circulating endothelial progenitor cells (EPCs) has introduced new possibilities for cell-based treatments for stroke. We tested the angiogenic gene expression of outgrowth endothelial cells (OECs), an EPC subtype capable to shape vessel structures. OECs (at colony or mature stages) from ischemic stroke patients (n=8) were characterized using the RT 2 Profiler TM human angiogenesis PCR Array, and human microvascular endothelial cells (hCMEC/D3) were used as an expression reference of endothelial cells. Colony-OECs showed higher expression of CCL2, ID3, IGF-1, MMP9, TGFBR1, TNFAIP2, TNF and TGFB1. However, BAI-1, NRP2, THBS1, MMP2 and VEGFC expression was increased in mature-OECs (p<0. 05). ID3 (p=0. 008) and TGFBR1 (p=0. 03) genes remained significantly overexpressed in colony-OECs compared to mature-OECs or hCMEC/D3. MMP9 levels were significantly increased in colony-OECs (p=0. 025) compared to mature-OECs. Moreover, MMP-2, VEGF-C, THBS1 and NRP-2 gene expression was also significantly increased in mature-OECs compared to hCMEC/D3 (p<0. 05). Some of these genes were positively validated by RT-PCR. Our study shows that OECs from stroke patients present higher levels of pro-angiogenic factors at early stages, decreasing in mature OECs when they become more similar to mature microvascular endothelial cells.
Ajuts:	Instituto de Salud Carlos III PI10-00694
Nota:	Altres ajuts: AR is supported by the Miguel Servet programme (CP09/00265) from the Spanish Ministry of Health (Instituto de Salud Carlos III). This work has been funded by Instituto de Salud Carlos III, grant PI10/00694 and the Spanish stroke research network RENEVAS (RD06/0026/0010) co-financed by the European Regional Development Fund (ERDF). This work has been supported also by the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreements n° 201024 and n° 202213 (European Stroke Network).
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Ischemic stroke ; Angiogenesis ; Endothelial progenitor cells ; Gene expression ; Vasculogenesis
Publicat a:	Vascular Cell, Vol. 5 (february 2013) , p. 3, ISSN 2045-824X

DOI: 10.1186/2045-824X-5-3
PMID: 23388410

7 p, 1.2 MB

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