FluoroType MTB system for the detection of pulmonary tuberculosis
Obasanya, Joshua (Nigerian Centers for Disease Control, Abuja, Nigeria)
Lawson, Lovett (Zankli Medical Center, Abuja, Nigeria)
Edwards, Thomas (Liverpool School of Tropical Medicine)
Olanrewaju, Oladimeji (Zankli Medical Center, Abuja, Nigeria)
Madukaji, Laura (Bingham University, Karu, Nigeria)
Dacombe, Russell (Liverpool School of Tropical Medicine)
Domínguez, José (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Molina-Moya, Barbara (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Adams, Emily (Liverpool School of Tropical Medicine)
Cuevas, Luis E.. (Liverpool School of Tropical Medicine)
Universitat Autònoma de Barcelona

Data:	2017
Resum:	Diagnosis continues to be a major barrier for the control of tuberculosis (TB), especially in low- and middle-income countries (LMIC) [1]. The number of platforms for the molecular diagnosis of TB have increased in recent years and they can provide test results more rapidly than culture. Molecular assays are increasingly being used as alternative or adjunct methods to culture and smear microscopy, and modern systems seek to partially or fully automate the DNA extraction and amplification steps, increasing their suitability for resource-limited laboratories. One of these platforms, the GeneXpert MTB/RIF (Cepheid, USA), has a sensitivity of roughly 85% compared to culture [2] and has seen significant uptake in developing countries [3]. However, as a fully closed system, the DNA extracted during the process cannot be used for further downstream drug susceptibility testing (DST), which is crucial for patients with suspected drug-resistant TB. FluoroType MTB is a sensitive test for TB but specificity is low compared with fully integrated molecular systems.
Nota:	Altres ajuts: The authors would like to acknowledge Hain Lifescience (Germany) for their provision of a FluoroCycler and sufficient FluoroType MTB assays to carry out the study. Hain Lifescience had no influence on the study design, data analysis or preparation of the manuscript. Funding information for this article has been deposited with the Crossref Funder Registry
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Diagnosis ; Tuberculosis ; Pulmonary tuberculosis ; Nucleic acid amplification tests ; Sensitivity ; Specificity
Publicat a:	ERJ Open Research, Vol. 3 (may 2017) , ISSN 2312-0541

DOI: 10.1183/23120541.00113-2016
PMID: 28491868

3 p, 281.9 KB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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