Web of Science: 5 citations, Scopus: 5 citations, Google Scholar: citations
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy
Roura, Santiago (Institut Germans Trias i Pujol. ICREC)
Gálvez Montón, Carolina (Institut Germans Trias i Pujol. ICREC)
de Gonzalo Calvo, David (Institut d'Investigació Biomèdica Sant Pau)
Valero, Ana Gámez (Institut Germans Trias i Pujol)
Gastelurrutia, Paloma (Institut Germans Trias i Pujol. ICREC)
Revuelta López, Elena (Institut Germans Trias i Pujol. ICREC)
Prat Vidal, Cristina (Institut Germans Trias i Pujol. ICREC)
Soler Botija, Carolina (Institut Germans Trias i Pujol. ICREC)
Llucià Valldeperas, Aida (Institut Germans Trias i Pujol. ICREC)
Perea Gil, Isaac (Institut Germans Trias i Pujol. ICREC)
Iborra Egea, Oriol (Institut Germans Trias i Pujol. ICREC)
Borràs, Francesc E. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lupón, Josep (Universitat Autònoma de Barcelona. Departament de Medicina)
Llorente Cortés, Vicenta (Institut d'Investigació Biomèdica Sant Pau)
Bayes Genis, Antoni (Universitat Autònoma de Barcelona. Departament de Medicina)

Date: 2017
Abstract: Idiopathic dilated cardiomyopathy () is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low‐density lipoprotein receptor‐related protein 1 (1) has been linked to the underlying molecular basis of the disease. This study compared circulating levels of soluble 1 (1) in patients and healthy controls and elucidated whether 1 is exported out of the myocardium through extracellular vesicles (s) to gain a better understanding of the pathogenesis of the disease. 1 α chain expression was analysed in samples collected from the left ventricles of explanted hearts using immunohistochemistry. 1 concentrations were determined in platelet‐free plasma by enzyme‐linked immunosorbent assay. Plasma‐derived s were extracted by size‐exclusion chromatography () and characterized by nanoparticle tracking analysis and cryo‐transmission electron microscopy. The distributions of vesicular (9, 81) and myocardial (caveolin‐3) proteins and 1 α chain were assessed in fractions by flow cytometry. 1 α chain was preferably localized to blood vessels in compared to control myocardium. Circulating 1 was increased in patients. 9‐ and 81‐positive fractions enriched with membrane vesicles with the expected size and morphology were isolated from both groups. The 1 α chain was not present in these fractions, which were also positive for caveolin‐3. The increase in circulating 1 in patients may be clinically valuable. Although s do not contribute to higher 1 levels in , a comprehensive analysis of content would provide further insights into the search for novel blood markers.
Note: Altres ajuts: Fundació La MARATÓ de TV3 (201502, 201516, 201521_10); Fundació Daniel Bravo Andreu; Sociedad Espanola de Cardiologıa; Societat Catalana de Cardiologia; Generalitat de Catalunya (SGR 2014, CERCA Programme); Fundació Bancaria La Caixa; Red de Terapia Celular TerCel (RD16/0011/0006); CIBER Cardiovascular (CB16/11/00403)
Note: Número d'acord de subvenció MICINN/SAF2014-59892-R
Note: Número d'acord de subvenció MINECO/JCI-2012-14025
Note: Número d'acord de subvenció ISCIII/FIS PI14-01682
Note: Número d'acord de subvenció ISCIII/FIS PI14-01729
Note: Número d'acord de subvenció ISCIII/CD14-00109
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Biomarker ; Idiopathic dilated cardiomyopathy ; Extracellular vesicles ; Slrp1 ; Size‐exclusion chromatography
Published in: Journal of Cellular and Molecular Medicine, Vol. 21 (may 2017) , p. 3000-3009, ISSN 1582-4934

PMID: 28557183
DOI: 10.1111/jcmm.13211


10 p, 723.5 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació Biomèdica Sant Pau
Articles > Research articles
Articles > Published articles

 Record created 2018-02-08, last modified 2019-05-16



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