Web of Science: 5 citations, Scopus: 5 citations, Google Scholar: citations
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy
Roura, Santiago (Institut Germans Trias i Pujol. ICREC)
Gálvez Montón, Carolina (Institut Germans Trias i Pujol. ICREC)
de Gonzalo Calvo, David (Institut d'Investigació Biomèdica Sant Pau)
Valero, Ana Gámez (Institut Germans Trias i Pujol)
Gastelurrutia, Paloma (Institut Germans Trias i Pujol. ICREC)
Revuelta López, Elena (Institut Germans Trias i Pujol. ICREC)
Prat Vidal, Cristina (Institut Germans Trias i Pujol. ICREC)
Soler Botija, Carolina (Institut Germans Trias i Pujol. ICREC)
Llucià Valldeperas, Aida (Institut Germans Trias i Pujol. ICREC)
Perea Gil, Isaac (Institut Germans Trias i Pujol. ICREC)
Iborra Egea, Oriol (Institut Germans Trias i Pujol. ICREC)
Borràs, Francesc E. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lupón, Josep (Universitat Autònoma de Barcelona. Departament de Medicina)
Llorente Cortés, Vicenta (Institut d'Investigació Biomèdica Sant Pau)
Bayes Genis, Antoni (Universitat Autònoma de Barcelona. Departament de Medicina)
Universitat Autònoma de Barcelona

Date: 2017
Abstract: Idiopathic dilated cardiomyopathy () is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low-density lipoprotein receptor-related protein 1 (1) has been linked to the underlying molecular basis of the disease. This study compared circulating levels of soluble 1 (1) in patients and healthy controls and elucidated whether 1 is exported out of the myocardium through extracellular vesicles (s) to gain a better understanding of the pathogenesis of the disease. 1 α chain expression was analysed in samples collected from the left ventricles of explanted hearts using immunohistochemistry. 1 concentrations were determined in platelet-free plasma by enzyme-linked immunosorbent assay. Plasma-derived s were extracted by size-exclusion chromatography () and characterized by nanoparticle tracking analysis and cryo-transmission electron microscopy. The distributions of vesicular (9, 81) and myocardial (caveolin-3) proteins and 1 α chain were assessed in fractions by flow cytometry. 1 α chain was preferably localized to blood vessels in compared to control myocardium. Circulating 1 was increased in patients. 9- and 81-positive fractions enriched with membrane vesicles with the expected size and morphology were isolated from both groups. The 1 α chain was not present in these fractions, which were also positive for caveolin-3. The increase in circulating 1 in patients may be clinically valuable. Although s do not contribute to higher 1 levels in , a comprehensive analysis of content would provide further insights into the search for novel blood markers.
Note: Altres ajuts: Fundació La MARATÓ de TV3 (201502, 201516, 201521_10); Fundació Daniel Bravo Andreu; Sociedad Espanola de Cardiologıa; Societat Catalana de Cardiologia; Generalitat de Catalunya (SGR 2014, CERCA Programme); Fundació Bancaria La Caixa; Red de Terapia Celular TerCel (RD16/0011/0006); CIBER Cardiovascular (CB16/11/00403)
Note: Número d'acord de subvenció MICINN/SAF2014-59892-R
Note: Número d'acord de subvenció MINECO/JCI-2012-14025
Note: Número d'acord de subvenció ISCIII/FIS PI14-01682
Note: Número d'acord de subvenció ISCIII/FIS PI14-01729
Note: Número d'acord de subvenció ISCIII/CD14-00109
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Biomarker ; Idiopathic dilated cardiomyopathy ; Extracellular vesicles ; Slrp1 ; Size-exclusion chromatography
Published in: Journal of Cellular and Molecular Medicine, Vol. 21 (may 2017) , p. 3000-3009, ISSN 1582-4934

DOI: 10.1111/jcmm.13211
PMID: 28557183


10 p, 723.5 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació Biomèdica Sant Pau
Articles > Research articles
Articles > Published articles

 Record created 2018-02-08, last modified 2020-11-12



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