BPSL1626 : reverse and structural vaccinology reveal a novel candidate for vaccine design against Burkholderia pseudomallei
Capelli, Riccardo (Center for Complexity and Biosystems and Dipartimento di Fisica, Università degli Studi di Milano)
Peri, Claudio (Consiglio Nazionale delle Ricerche. Istituto di Chimica del Riconoscimento Molecolare)
Villa, Riccardo (Department of Biosciences, Università degli Studi di Milano)
Nithichanon, Arnone (Khon Kaen University. Centre for Research and Development of Medical Diagnostic Laboratories)
Conchillo-Solé, Oscar (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Yero, Daniel (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Gagni, Paola (Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche)
Chiari, Marcella (Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche)
Lertmemongkolchai, Ganjana (Center for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University)
Cretich, Marina (Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche)
Daura i Ribera, Xavier (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Bolognesi, Martino (Università degli Studi di Milano. Centro di Eccellenza Interdisciplinare Materiali e Interfacce Nanostrutturati)
Colombo, Giorgio (Consiglio Nazionale delle Ricerche. Istituto di Chimica del Riconoscimento Molecolare)
Gourlay, Louise J. (Università degli Studi di Milano. Centro di Eccellenza Interdisciplinare Materiali e Interfacce Nanostrutturati)

Fecha:	2018
Resumen:	Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that identified a panel of 104 candidate antigens from the Gram-negative bacterial pathogen Burkholderia pseudomallei, which is responsible for the disease melioidosis. B. pseudomallei can cause fatal sepsis in endemic populations in the tropical regions of the world and treatment with antibiotics is mostly ineffective. With the aim of identifying potential vaccine candidates, we report the experimental validation of predicted antigen and type I fimbrial subunit, BPSL1626, which we show is able to recognize and bind human antibodies from the sera of Burkholderia infected patients and to stimulate T-lymphocytes in vitro. The prerequisite for a melioidosis vaccine, in fact, is that both antibody- and cell-mediated immune responses must be triggered. In order to reveal potential antigenic regions of the protein that may aid immunogen re-design, we also report the crystal structure of BPSL1626 at 1. 9 Å resolution on which structure-based epitope predictions were based. Overall, our data suggest that BPSL1626 and three epitope regions here-identified can represent viable candidates as potential antigenic molecules.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Burkholderia ; BPSL1626 antigen ; Melioidosis ; Reverse vaccinology ; Crystal structure ; In silico epitope predictions ; Type I fimbrial subunit
Publicado en:	Antibodies, Vol. 7 (2018) , p. 1-14

DOI: 10.3390/antib7030026
PMID: 31544878

14 p, 7.6 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Biotecnología y de Biomedicina (IBB)
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