A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs
Cho, In-Cheol (Rural Development Administration. National Institute of Animal Science)
Park, Hee-Bok (Kongju National University. College of Industrial Sciences. Department of Animal Resources Science)
Ahn, Jin Seop (Korea Research Institute of Bioscience and Biotechnology. Biotherapeutics Translational Research Center)
Han, Sang-Hyun (Jeju National University. Educational Science Research Institute)
Lee, JaeBong (Chonbuk National University. Korea Zoonosis Research Institute)
Lim, Hyun-Tae (Gyeongsang National University. College of Agriculture and Life Sciences)
Yoo, Chae-Kyoung (Gyeongsang National University. Institute of Agriculture and Life Science.)
Jung, Eun-Ji (Bio-Medical Science Co., Ltd)
Kim, Dong-Hwan (Korea Research Institute of Bioscience and Biotechnology. Biotherapeutics Translational Research Center)
Sun, Wu-Sheng (Korea Research Institute of Bioscience and Biotechnology. Biotherapeutics Translational Research Center)
Ramayo-Caldas, Yuliaxis (Institut de Recerca i Tecnologia Agroalimentàries)
Kim, Sang-Geum (Rural Development Administration. National Institute of Animal Science)
Kang, Yong-Jun (Rural Development Administration. National Institute of Animal Science)
Kim, YooKyung (Jeju National University. Educational Science Research Institute)
Shin, Hyun-Sook (Rural Development Administration. National Institute of Animal Science)
Seong, Pil-Nam (Rural Development Administration. National Institute of Animal Science)
Hwang, In-Sul (Rural Development Administration. National Institute of Animal Science)
Park, Beom-Young (Rural Development Administration. National Institute of Animal Science)
Hwang, Seongsoo (Rural Development Administration. National Institute of Animal Science)
Lee, Sung-Soo (Rural Development Administration. National Institute of Animal Science)
Ryu, Youn-Chul (Jeju National University. Division of Biotechnology. SARI)
Lee, Jun-Heon (Chungnam National University. Division of Animal and Dairy Science)
Ko, MoonSuck (Rural Development Administration. National Institute of Animal Science)
Lee, Kichoon (Ohio State University. College of Food Agricultural and Environmental Sciences)
Andersson, Göran (Swedish University of Agricultural Sciences. Department of Animal Breeding and Genetics)
Perez-Enciso, Miguel (Centre de Recerca en Agrigenòmica)
Lee, JeongWoong (Korea Research Institute of Bioscience and Biotechnology. Biotherapeutics Translational Research Center)

Date:	2019
Abstract:	Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488. 1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.
Grants:	Ministerio de Economía y Competitividad AGL2016-78709-R
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Adipogenesis ; Adipose Tissue ; Animals ; Breeding ; Cytoskeletal Proteins ; Gene Expression Regulation ; Genome-Wide Association Study ; Genotype ; Meat ; Mice ; Mice, Transgenic ; Muscle Fibers, Skeletal ; Muscle, Skeletal ; Myosin Heavy Chains ; Myosins ; Nucleotide Motifs ; Sus scrofa ; Swine
Published in:	PLoS Genetics, Vol. 15, Núm. 10 (October 2019) , art. e1008279, ISSN 1553-7404

DOI: 10.1371/journal.pgen.1008279
PMID: 31603892

26 p, 3.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
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