The ATM signaling cascade promotes recombination-dependent pachytene arrest in mouse spermatocytes
Pacheco, Sarai (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Marcet-Ortega, Marina (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Lange, Julian (Memorial Sloan Kettering Cancer Center)
Jasin, Maria (Memorial Sloan Kettering Cancer Center)
Keeney, Scott (Memorial Sloan Kettering Cancer Center)
Roig, Ignasi (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Date:	2015
Abstract:	Most mutations that compromise meiotic recombination or synapsis in mouse spermatocytes result in arrest and apoptosis at the pachytene stage of the first meiotic prophase. Two main mechanisms are thought to trigger arrest: one independent of the double-strand breaks (DSBs) that initiate meiotic recombination, and another activated by persistent recombination intermediates. Mechanisms underlying the recombination-dependent arrest response are not well understood, so we sought to identify factors involved by examining mutants deficient for TRIP13, a conserved AAA+ ATPase required for the completion of meiotic DSB repair. We find that spermatocytes with a hypomorphic Trip13 mutation (Trip13<sup.
Grants:	Ministerio de Ciencia e Innovación BES-2011-045381 Ministerio de Ciencia e Innovación BFU2010-18965 Agència de Gestió d'Ajuts Universitaris i de Recerca 2010/BE100718
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Adenosine triphosphatases ; Animals ; Apoptosis ; Ataxia telangiectasia mutated proteins ; Cell cycle checkpoints ; Cell cycle proteins ; Checkpoint kinase 2 ; Chromosome pairing ; DNA breaks, Double-Stranded ; DNA repair ; DNA repair enzymes ; DNA-binding proteins ; Male ; Mice ; Mutation ; Nuclear proteins ; Pachytene stage ; Recombination, Genetic ; Signal transduction ; Spermatocytes
Published in:	PLOS genetics, Vol. 11, issue 3 (2015) , art. e1005017, ISSN 1553-7404

DOI: 10.1371/journal.pgen.1005017
PMID: 25768017

27 p, 21.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles