Interleukin-6 Derived from the Central Nervous System May Influence the Pathogenesis of Experimental Autoimmune Encephalomyelitis in a Cell-Dependent Manner
Sanchis, Paula (Universitat Autònoma de Barcelona. Institut de Neurociències)
Fernandez-Gayol, Olaya (Universitat Autònoma de Barcelona. Institut de Neurociències)
Comes, Gemma (Universitat Autònoma de Barcelona. Institut de Neurociències)
Escrig, Anna (Universitat Autònoma de Barcelona. Institut de Neurociències)
Giralt, Mercedes (Universitat Autònoma de Barcelona. Institut de Neurociències)
Palmiter, Richard D. (Department of Biochemistry, Genome Sciences, and Howard Hughes Medical Institute, University of Washington)
Hidalgo Pareja, Juan (Universitat Autònoma de Barcelona. Institut de Neurociències)

Date:	2020
Abstract:	BACKGROUND: Interleukin-6 (IL-6) is a pleiotropic and multifunctional cytokine that plays a critical role in induction of experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis (MS). Although EAE has always been considered a peripherally elicited disease, Il6 expression exclusively within central nervous system is sufficient to induce EAE development. Neurons, astrocytes, and microglia can secrete and respond to IL-6. METHODS: To dissect the relevance of each cell source for establishing EAE, we generated and immunized conditional Il6 knockout mice for each of these cell types with myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) peptide dissolved in complete Freund's adjuvant (CFA) and supplemented with Mycobacterium tuberculosis. RESULTS AND CONCLUSIONS: The combined results reveal a minor role for Il6 expression in both astrocytes and microglia for symptomatology and neuropathology of EAE, whereas neuronal Il6 expression was not relevant for the variables analyzed.
Grants:	Ministerio de Economía y Competitividad BES-2015-071959 Ministerio de Educación, Cultura y Deporte FPU2012/00365 Ministerio de Economía y Competitividad SAF2014-56546-R Agencia Estatal de Investigación RTI2018-101105-B-I00
Note:	Altres ajuts: La Marató de TV3 20142210
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Conditional IL-6 knockout ; Experimental autoimmune encephalomyelitis ; MOG35-55peptide ; Neuroinflammation
Published in:	Cells, Vol. 9 Núm. 2 (2 2020) , ISSN 2073-4409

DOI: 10.3390/cells9020330
PMID: 32023844

18 p, 4.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles