Blood parameters as biomarkers in a Salmonella spp. disease model of weaning piglets
Barba-Vidal, Emili (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Buttow Roll, Victor Fernando (Federal University of Pelotas. Department of Animal Science)
García Manzanilla, Edgar (Animal and Grassland Research and Innovation Centre. Pig Development Department)
Torrente Artero, Carlos (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Moreno Muñoz, José Antonio (Laboratorios Ordesa)
Pérez Hernández, José Francisco (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Martín Orúe, Susana M. (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Data:	2017
Resum:	The weaning pig is used as an experimental model to assess the impact of diet on intestinal health. Blood parameters (BP) are considered a useful tool in humans, but there is very scarce information of such indicators in the weaning pig. The objective of the present study is to evaluate the use of different BP as indicators in an experimental model of salmonellosis. Seventy-two 28-day-old piglets were divided into four groups in a 2x2 factorial arrangement, with animals receiving or not a probiotic combination based on B. infantis IM1® and B. lactis BPL6 (10 9 colony forming units (cfu)/d) and orally challenged or not a week later with Salmonella Typhimurium (5x10 8 cfu). Blood samples of one animal per pen (N = 24) were taken four days post-inoculation for the evaluation of different BP using an I-stat® System and of plasmatic concentrations of zinc, iron and copper. Results reported marginal deficiencies of zinc in piglets at weaning. Moreover, plasmatic zinc, copper and iron presented good correlations with weight gain (r 0. 57, r -0. 67, r 0. 54 respectively; P < 0. 01). Blood electrolytes (Na +, Cl - and K +) decreased (P < 0. 01) only when the performance of the animals was seriously compromised and clinical symptoms were more apparent. Acid-base balance parameters such as HCO3 -, TCO2 and BE significantly correlated with weight gain, but only in the challenged animals (r -0. 54, r -0. 55, and r -0. 51, respectively; P < 0. 05), suggesting metabolic acidosis depending on Salmonella infection. Glucose was affected by the challenge (P = 0. 040), while Htc and Hgb increased with the challenge and decreased with the probiotic (P < 0. 05). Furthermore, correlations of Glu, Htc and Hgb with weight gain were observed (P < 0. 05). Overall, BP could be regarded as simple, useful indexes to assess performance and health of weaning piglets.
Ajuts:	Ministerio de Educación y Ciencia AGL 2012-31924
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 12 (october 2017) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0186781
PMID: 29073182

16 p, 2.1 MB

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