Proteomic comparison between non-purified cerebrospinal fluid and cerebrospinal fluid-derived extracellular vesicles from patients with Alzheimer's, Parkinson's and Lewy body dementia
Hirschberg, Yael (University of Antwerp)
Valle-Tamayo, Natalia (Institut d'Investigació Biomèdica Sant Pau)
Dols Icardo, Oriol (Institut d'Investigació Biomèdica Sant Pau)
Engelborghs, Sebastiaan (University of Antwerp)
Buelens, Bart (Flemish Institute for Technological Research (VITO))
Vandenbroucke, Roosmarijn (Ghent University)
Vermeiren, Yannick (University of Antwerp)
Boonen, Kurt (University of Antwerp)
Mertens, Inge (University of Antwerp)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	Dementia is a leading cause of death worldwide, with increasing prevalence as global life expectancy increases. The most common neurodegenerative disorders are Alzheimer's disease (AD), dementia with Lewy bodies (DLB) and Parkinson's disease dementia (PDD). With this study, we took an in-depth look at the proteome of the (non-purified) cerebrospinal fluid (CSF) and the CSF-derived extracellular vesicles (EVs) of AD, PD, PD-MCI (Parkinson's disease with mild cognitive impairment), PDD and DLB patients analysed by label-free mass spectrometry. This has led to the discovery of differentially expressed proteins that may be helpful for differential diagnosis. We observed a greater number of differentially expressed proteins in CSF-derived EV samples (N = 276) compared to non-purified CSF (N = 169), with minimal overlap between both datasets. This finding suggests that CSF-derived EV samples may be more suitable for the discovery phase of a biomarker study, due to the removal of more abundant proteins, resulting in a narrower dynamic range. As disease-specific markers, we selected a total of 39 biomarker candidates identified in non-purified CSF, and 37 biomarker candidates across the different diseases under investigation in the CSF-derived EV data. After further exploration and validation of these proteins, they can be used to further differentiate between the included dementias and may offer new avenues for research into more disease-specific pharmacological therapeutics.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biomarkers ; Cerebrospinal fluid ; Dementia ; Extracellular vesicles ; Mass spectrometry ; Proteomics
Publicat a:	Journal of extracellular vesicles, Vol. 12 Núm. 12 (december 2023) , p. 12383, ISSN 2001-3078

DOI: 10.1002/jev2.12383
PMID: 38082559

22 p, 2.2 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut de Recerca Sant Pau
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