Osmostress-Induced Apoptosis in Xenopus Oocytes : Role of Stress Protein Kinases, Calpains and Smac/DIABLO
Ben Messaoud, Nabil (Universitat Autònoma de Barcelona. Institut de Neurociències)
Yue, Jicheng (Universitat Autònoma de Barcelona. Institut de Neurociències)
Valent, Daniel (Universitat Autònoma de Barcelona. Institut de Neurociències)
Katzarova, Ilina (Universitat Autònoma de Barcelona. Institut de Neurociències)
López Blanco, José Manuel (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Date:	2015
Abstract:	Hyperosmotic shock induces cytochrome c release and capase-3 activation in Xenopus oocytes, but the regulators and signaling pathways involved are not well characterized. Here we show that hyperosmotic shock induces rapid calpain activation and high levels of Smac/DIABLO release from the mitochondria before significant amounts of cytochrome c are released to promote caspase-3 activation. Calpain inhibitors or EGTA microinjection delays osmostress-induced apoptosis, and blockage of Smac/DIABLO with antibodies markedly reduces cytochrome c release and caspase-3 activation. Hyperosmotic shock also activates the p38 and JNK signaling pathways very quickly. Simultaneous inhibition of both p38 and JNK pathways reduces osmostress-induced apoptosis, while sustained activation of these kinases accelerates the release of cytochrome c and caspase-3 activation. Therefore, at least four different pathways early induced by osmostress converge on the mitochondria to trigger apoptosis. Deciphering the mechanisms of hyperosmotic shock-induced apoptosis gives insight for potential treatments of human diseases that are caused by perturbations in fluid osmolarity.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Xenopus oocytes ; Apoptosis ; Mitochondria ; Oocytes ; Osmotic shock ; Phosphorylation ; Protein kinases ; Cytosol
Published in:	PloS one, Vol. 10, Num. 4 (2015) , p. 1-19, ISSN 1932-6203

DOI: 10.1371/journal.pone.0124482
PMID: 25866890

19 p, 9.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles