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| Home > Articles > Published articles > Analysis of porcine IGF2 gene expression in adipose tissue and its effect on fatty acid composition |
(Centre de Recerca en Agrigenòmica) (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Genètica i Millora Animal) (Centre de Recerca en Agrigenòmica) (Centre de Recerca en Agrigenòmica) (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya). Departamento de Mejora Genética Animal) (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya). Departamento de Mejora Genética Animal) (Centre de Recerca en Agrigenòmica)| Date: | 2019 |
| Abstract: | IGF2:g. 3072G>A polymorphism has been described as the causal mutation of a maternally imprinted QTL for muscle growth and fat deposition in pigs. The objective of the current work was to study the association between the IGF2:g. 3072G>A polymorphism and the IGF2 gene expression and its effect on fatty acid composition in adipose tissue in different pig genetic backgrounds. A cis-eQTL region associated with the IGF2 mRNA expression in adipose tissue was identified in an eGWAS with 355 animals. The IGF2 gene was located in this genomic interval and IGF2g. 3072G>A was the most significant SNP, explaining a 25% of the gene expression variance. Significant associations between IGF2:g. 3072G>A polymorphism and oleic (C18:1(n-9); p-value = 4. 18x10), hexadecanoic (C16:1(n-9); p-value = 4. 04x10), linoleic (C18:2(n-6); p-value = 6. 44x10), α-linoleic (C18:3(n-3); p-value = 3. 30x10), arachidonic (C20:4(n-6); p-value = 9. 82x10) FAs and the MUFA/PUFA ratio (p-value = 2. 51x10) measured in backfat were identified. Animals carrying the A allele showed an increase in IGF2 gene expression and higher PUFA and lower MUFA content. However, in additional studies was observed that there could be other proximal genetic variants affecting FA composition in adipose tissue. Finally, no differences in the IGF2 gene expression in adipose tissue were found between heterozygous animals classified according to the IGF2:g. 3072G>A allele inherited from the father (AG or AG). However, pyrosequencing analysis revealed that there is imprinting of the IGF2 gene in muscle and adipose tissues, with stronger differences among the paternally and maternally inherited alleles in muscle. Our results suggested that IGF2:g. 3072G>A polymorphism plays an important role in the regulation of IGF2 gene expression and can be involved in the fatty acid composition in adipose tissue. In both cases, further studies are still needed to deepen the mechanism of regulation of IGF2 gene expression in adipose tissue and the IGF2 role in FA composition. |
| Grants: | Ministerio de Economía y Competitividad AGL2014-56369-C2 Ministerio de Economía y Competitividad AGL2017-82641-R Ministerio de Economía y Competitividad RYC-2013-12573 Ministerio de Economía y Competitividad SEV-2015-0533 |
| Note: | Altres ajuts: Ph.D grant from the Generalitat de Catalunya (ECO/1788/2014) i CERCA Programme/Generalitat de Catalunya |
| Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Language: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Subject: | Adipose tissue ; Alleles ; Animals ; Fatty acids ; Fatty acids, Monounsaturated ; Fatty acids, Unsaturated ; Gene expression regulation ; Genome-wide association study ; Insulin-like growth factor II ; Polymorphism, Single nucleotide ; Quantitative trait loci ; Swine |
| Published in: | PloS one, Vol. 14, Issue 8 (August 2019) , p. e0220708, ISSN 1932-6203 |
