Disease-associated mutations impacting BC-loop flexibility trigger long-range transthyretin tetramer destabilization and aggregation
Esperante, Sebastián A. (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Varejão, Nathalia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Pinheiro, Francisca (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Sant'Anna, Ricardo (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Luque-Ortega, Juan Román (Centro de Investigaciones Biológicas (Madrid))
Alfonso, Carlos (Centro de Investigaciones Biológicas (Madrid))
Sora, Valentina (Technical University of Denmark. Cancer Systems Biology, Health and Technology Department)
Papaleo, Elena (Technical University of Denmark. Cancer Systems Biology, Health and Technology Department)
Rivas, Germán (Centro de Investigaciones Biológicas (Madrid))
Reverter i Cendrós, David (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ventura, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Date:	2021
Abstract:	Hereditary transthyretin amyloidosis (ATTR) is an autosomal dominant disease characterized by the extracellular deposition of the transport protein transthyretin (TTR) as amyloid fibrils. Despite the progress achieved in recent years, understanding why different TTR residue substitutions lead to different clinical manifestations remains elusive. Here, we studied the molecular basis of disease-causing missense mutations affecting residues R34 and K35. R34G and K35T variants cause vitreous amyloidosis, whereas R34T and K35N mutations result in amyloid polyneuropathy and restrictive cardiomyopathy. All variants are more sensitive to pH-induced dissociation and amyloid formation than the wild-type (WT)-TTR counterpart, specifically in the variants deposited in the eyes amyloid formation occurs close to physiological pHs. Chemical denaturation experiments indicate that all the mutants are less stable than WT-TTR, with the vitreous amyloidosis variants, R34G and K35T, being highly destabilized. Sequence-induced stabilization of the dimer-dimer interface with T119M rendered tetramers containing R34G or K35T mutations resistant to pH-induced aggregation. Because R34 and K35 are among the residues more distant to the TTR interface, their impact in this region is therefore theorized to occur at long range. The crystal structures of double mutants, R34G/T119M and K35T/T119M, together with molecular dynamics simulations indicate that their strong destabilizing effect is initiated locally at the BC loop, increasing its flexibility in a mutation-dependent manner. Overall, the present findings help us to understand the sequence-dynamic-structural mechanistic details of TTR amyloid aggregation triggered by R34 and K35 variants and to link the degree of mutation-induced conformational flexibility to protein aggregation propensity.
Grants:	Ministerio de Ciencia e Innovación PID2019-105017RB-100 Agencia Estatal de Investigación PID2019-104544GB-I00
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Transthyretin ; Amyloid ; Aggregation ; Protein structure ; Protein stability ; Molecular dynamics ; ERAD, endoplasmic reticulum-associated degradation ; FAC, familial amyloid cardiomyopathy ; FAP, familial amyloid polyneuropathy ; MD, molecular dynamics ; RMSD, root mean square deviation ; RMSF, root mean square fluctuation ; TEM, transmission electron microscopy ; TTR, transthyretin ; WT, wild-type
Published in:	Journal of biological chemistry, Vol. 297, Issue 3 (September 2021) , art. 101039, ISSN 1083-351X

DOI: 10.1016/j.jbc.2021.101039
PMID: 34343569

14 p, 2.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles