Extending MeCP2 interactome : canonical nucleosomal histones interact with MeCP2
Ortega-Alarcon, David (Instituto de Investigación Sanitaria de Aragón)
Claveria-Gimeno, Rafael (Certest Biotec (Saragossa))
Vega, Sonia (Universidad de Zaragoza)
Kalani, Ladan (University of Victoria (Canadà))
Jorge-Torres, Olga Caridad (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Esteller, M. (Universitat de Barcelona. Departament de Ciències Fisiològiques)
Ausio, Juan (University of Victoria (Canadà))
Abián, Olga (Universidad de Zaragoza. Departamento de Bioquímica y Biología Molecular y Celular)
Velázquez-Campoy, Adrián (Universidad de Zaragoza. Departamento de Bioquímica y Biología Molecular y Celular)

Date:	2024
Abstract:	MeCP2 is a general regulator of transcription involved in the repression/activation of genes depending on the local epigenetic context. It acts as a chromatin regulator and binds with exquisite specificity to gene promoters. The set of epigenetic marks recognized by MeCP2 has been already established (mainly, cytosine modifications in CpG and CpA), as well as many of the constituents of its interactome. We unveil a new set of interactions for MeCP2 with the four canonical nucleosomal histones. MeCP2 interacts with high affinity with H2A, H2B, H3 and H4. In addition, Rett syndrome associated mutations in MeCP2 and histone epigenetic marks modulate these interactions. Given the abundance and the structural/functional relevance of histones and their involvement in epigenetic regulation, this new set of interactions and its modulating elements provide a new addition to the 'alphabet' for this epigenetic reader.
Grants:	Agencia Estatal de Investigación PID2021-127296OB-I00 Instituto de Salud Carlos III PI21/00394
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Animals ; Epigenesis, Genetic ; Histones ; Humans ; Methyl-CpG-Binding Protein 2 ; Mutation ; Nucleosomes ; Protein Binding ; Rett Syndrome
Published in:	Nucleic acids research, Vol. 52 Núm. 7 (24 2024) , p. 3636-3653, ISSN 1362-4962

DOI: 10.1093/nar/gkae051
PMID: 38321951

18 p, 3.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP) > Josep Carreras Leukaemia Research Institute
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Articles > Published articles