Web of Science: 9 cites, Scopus: 10 cites, Google Scholar: cites,
Role of AMPK signalling pathway during compensatory growth in pigs
Ballester Devis, Maria (Institut de Recerca i Tecnologia Agroalimentàries)
Amills i Eras, Marcel (Centre de Recerca en Agrigenòmica)
González-Rodríguez, Olga (Institut de Recerca i Tecnologia Agroalimentàries)
Cardoso, Tainã Figueiredo (Centre de Recerca en Agrigenòmica)
Pascual, Mariam (Institut de Recerca i Tecnologia Agroalimentàries)
González Prendes, Rayner (Centre de Recerca en Agrigenòmica)
Panella Riera, Núria (Institut de Recerca i Tecnologia Agroalimentàries)
Díaz-Reviriego, Isabel (Institut de Recerca i Tecnologia Agroalimentàries)
Tibau, Joan (Institut de Recerca i Tecnologia Agroalimentàries)
Quintanilla, Raquel (Institut de Recerca i Tecnologia Agroalimentàries)
Universitat Autònoma de Barcelona

Data: 2018
Resum: Background: The molecular basis of compensatory growth in monogastric animals has not yet been fully explored. Herewith, in this study we aim to determine changes in the pig skeletal muscle transcriptome profile during compensatory growth following a feed restriction period. A RNA-Seq experiment was performed with a total of 24 females belonging to a Duroc commercial line. Half of the animals received either a restricted (RE) or ad libitum (AL) diet during the first fattening period (60-125 d of age). After that, all gilts were fed ad libitum for a further ~30 d until the age of ~155 d, when animals were slaughtered and samples of gluteus medius muscle were harvested to perform RNA-Seq analyses and intramuscular fat content determination. Results: During the period following food restriction, RE animals re-fed ad libitum displayed compensatory growth, showed better feed conversion rate and tended to deposit more subcutaneous fat than AL fed animals. Animals were slaughtered in the phase of accelerated growth, when RE animals had not completely compensated the performance of AL group, showing lower live and carcass weights. At intramuscular level, RE gilts showed a higher content of polyunsaturated fatty acids during the compensatory growth phase. The comparison of RE and AL expression profiles allowed the identification of 86 (logFold-Change > 1, p < 0. 05) differentially expressed (DE) genes. A functional categorization of these DE genes identified AMPK Signaling as the most significantly enriched canonical pathway. This kinase plays a key role in the maintenance of energy homeostasis as well as in the activation of autophagy. Among the DE genes identified as components of AMPK Signaling pathway, five out of six genes were downregulated in RE pigs. Conclusions: Animals re-fed after a restriction period exhibited a less oxidative metabolic profile and catabolic processes in muscle than animals fed ad libitum. The downregulation of autophagy observed in the skeletal muscle of pigs undergoing compensatory growth may constitute a mechanism to increase muscle mass thus ensuring an accelerated growth rate. These results reveal that the downregulation of AMPK Signaling plays an important role in compensatory growth in pigs.
Ajuts: Ministerio de Economía y Competitividad AGL2013-48742-C2-1-R
Ministerio de Economía y Competitividad AGL2013-48742-C2-2-R
Ministerio de Economía y Competitividad SEV-2015-0533
Ministerio de Economía y Competitividad RYC-2013-12573
Ministerio de Educación y Ciencia FPU12/00860
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Pig ; Feed restriction ; Compensatory growth ; Skeletal muscle ; RNA-Seq ; Autophagy ; Energy homeostasis ; AMPK
Publicat a: BMC genomics, Vol. 19 (September 2018) , art. 682, ISSN 1471-2164

DOI: 10.1186/s12864-018-5071-5
PMID: 30223793

12 p, 977.9 KB

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 Registre creat el 2019-11-13, darrera modificació el 2022-06-08

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