Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria
Pesarrodona Roches, Mireia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Fernández Caparrós, Yolanda (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Foradada Felip, Laia (Hospital Universitari Vall d'Hebron)
Sánchez Chardi, Alejandro (Universitat Autònoma de Barcelona. Servei de Microscòpia)
Conchillo-Solé, Oscar (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Xu, Zhikun (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Roldán, Mónica (Universitat Autònoma de Barcelona. Servei de Microscòpia)
Villegas Hernández, Sandra (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Schwartz, Simó (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Rinas, Ursula (Leibniz University of Hannover. Technical Chemistry & Life Science)
Daura i Ribera, Xavier (Institució Catalana de Recerca i Estudis Avançats)
Abasolo, Ibane (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2016
Abstract:	Biofabrication is attracting interest as a means to produce nanostructured functional materials because of its operational versatility and full scalability. Materials based on proteins are especially appealing, as the structure and functionality of proteins can be adapted by genetic engineering. Furthermore, strategies and tools for protein production have been developed and refined steadily for more than 30 years. However, protein conformation and therefore activity might be sensitive to production conditions. Here, we have explored whether the downstream strategy influences the structure and biological activities, in vitro and in vivo, of a self-assembling, CD44-targeted protein-only nanoparticle produced in Escherichia coli. This has been performed through the comparative analysis of particles built from soluble protein species or protein versions obtained by in vitro protein extraction from inclusion bodies, through mild, non-denaturing procedures. These methods have been developed recently as a convenient alternative to the use of toxic chaotropic agents for protein resolubilization from protein aggregates. The results indicate that the resulting material shows substantial differences in its physicochemical properties and its biological performance at the systems level, and that its building blocks are sensitive to the particular protein source.
Grants:	Ministerio de Economía y Competitividad BIO2013-41019-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-132 Fundació la Marató de TV3 2013-3930
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Nanoparticles ; Cell factories ; Recombinant proteins ; Bacteria ; Protein folding ; Drug delivery
Published in:	Biofabrication, Vol. 8 Núm 2 (Abril 2016) , p. 25001, ISSN 1758-5090

DOI: 10.1088/1758-5090/8/2/025001

Postprint 35 p, 1.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles